Natural heterogeneous catalysis with immobilised oxidase biocatalysts

Ashley P. Mattey, Jack J. Sangster, Jeremy I. Ramsden, Christopher Baldwin, William R. Birmingham, Rachel S. Heath, Antonio Angelastro, Nicholas J. Turner, Sebastian C. Cosgrove, Sabine L. Flitsch

Research output: Contribution to journalArticlepeer-review


The generation of immobilised oxidase biocatalysts allowing multifunctional oxidation of valuable chemicals using molecular oxygen is described. Engineered galactose oxidase (GOase) variants M1 and M3–5, an engineered choline oxidase (AcCO6) and monoamine oxidase (MAO-N D9) displayed long-term stability and reusability over several weeks when covalently attached on a solid support, outperforming their free counterparts in terms of stability (more than 20 fold), resistance to heat at 60 °C, and tolerance to neat organic solvents such as hexane and toluene. These robust heterogenous oxidation catalysts can be recovered after each reaction and be reused multiple times for the oxidation of different substrates
Original languageEnglish
Pages (from-to)19501-19505
JournalRSC Advances
Issue number33
Early online date21 May 2020
Publication statusE-pub ahead of print - 21 May 2020


Dive into the research topics of 'Natural heterogeneous catalysis with immobilised oxidase biocatalysts'. Together they form a unique fingerprint.

Cite this