NBCe2 exhibits a 3 HCO3-:1 Na+ stoichiometry in mouse choroid plexus epithelial cells

Ian D. Millar; Peter D. Brown

doi:10.1016/j.bbrc.2008.06.053

NBCe2 exhibits a 3 HCO3-:1 Na+ stoichiometry in mouse choroid plexus epithelial cells

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Abstract

The transport stoichiometry of the electrogenic sodium-bicarbonate cotransporter (SLC4A5 or NBCe2) in mouse choroid plexus was examined. Whole-cell recording methods measured the currents carried by the NBCe2, using experimental solutions determined to minimise the contributions of the other ion conductances present. Increases in outward current were observed when 21.2 mM HCO3- was added to the bath solution in the presence of Na+, but not N-methyl-d-glucamine. This HCO3--induced current was completely abolished by 500 μM 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid. The reversal potential for the HCO3--induced current was -95.1 ± 7.1 mV (n = 11), a value which corresponds to a NBCe2 transport stoichiometry of 3 HCO3- with 1 Na+. The NBCe2, with this stoichiometry, will mediate the efflux of HCO3- and Na+ from the cell into the cerebrospinal fluid at the apical membrane of the choroid plexus. © 2008 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	550-554
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	373
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.06.053
Publication status	Published - 5 Sept 2008

Keywords

Cerebrospinal fluid
Choroid plexus
Electrogenic
Epithelium
Sodium-bicarbonate cotransporter
Whole-cell path clamp

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10.1016/j.bbrc.2008.06.053

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title = "NBCe2 exhibits a 3 HCO3-:1 Na+ stoichiometry in mouse choroid plexus epithelial cells",

abstract = "The transport stoichiometry of the electrogenic sodium-bicarbonate cotransporter (SLC4A5 or NBCe2) in mouse choroid plexus was examined. Whole-cell recording methods measured the currents carried by the NBCe2, using experimental solutions determined to minimise the contributions of the other ion conductances present. Increases in outward current were observed when 21.2 mM HCO3- was added to the bath solution in the presence of Na+, but not N-methyl-d-glucamine. This HCO3--induced current was completely abolished by 500 μM 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid. The reversal potential for the HCO3--induced current was -95.1 ± 7.1 mV (n = 11), a value which corresponds to a NBCe2 transport stoichiometry of 3 HCO3- with 1 Na+. The NBCe2, with this stoichiometry, will mediate the efflux of HCO3- and Na+ from the cell into the cerebrospinal fluid at the apical membrane of the choroid plexus. {\textcopyright} 2008 Elsevier Inc. All rights reserved.",

keywords = "Cerebrospinal fluid, Choroid plexus, Electrogenic, Epithelium, Sodium-bicarbonate cotransporter, Whole-cell path clamp",

author = "Millar, {Ian D.} and Brown, {Peter D.}",

year = "2008",

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doi = "10.1016/j.bbrc.2008.06.053",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - NBCe2 exhibits a 3 HCO3-:1 Na+ stoichiometry in mouse choroid plexus epithelial cells

AU - Millar, Ian D.

AU - Brown, Peter D.

PY - 2008/9/5

Y1 - 2008/9/5

N2 - The transport stoichiometry of the electrogenic sodium-bicarbonate cotransporter (SLC4A5 or NBCe2) in mouse choroid plexus was examined. Whole-cell recording methods measured the currents carried by the NBCe2, using experimental solutions determined to minimise the contributions of the other ion conductances present. Increases in outward current were observed when 21.2 mM HCO3- was added to the bath solution in the presence of Na+, but not N-methyl-d-glucamine. This HCO3--induced current was completely abolished by 500 μM 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid. The reversal potential for the HCO3--induced current was -95.1 ± 7.1 mV (n = 11), a value which corresponds to a NBCe2 transport stoichiometry of 3 HCO3- with 1 Na+. The NBCe2, with this stoichiometry, will mediate the efflux of HCO3- and Na+ from the cell into the cerebrospinal fluid at the apical membrane of the choroid plexus. © 2008 Elsevier Inc. All rights reserved.

AB - The transport stoichiometry of the electrogenic sodium-bicarbonate cotransporter (SLC4A5 or NBCe2) in mouse choroid plexus was examined. Whole-cell recording methods measured the currents carried by the NBCe2, using experimental solutions determined to minimise the contributions of the other ion conductances present. Increases in outward current were observed when 21.2 mM HCO3- was added to the bath solution in the presence of Na+, but not N-methyl-d-glucamine. This HCO3--induced current was completely abolished by 500 μM 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid. The reversal potential for the HCO3--induced current was -95.1 ± 7.1 mV (n = 11), a value which corresponds to a NBCe2 transport stoichiometry of 3 HCO3- with 1 Na+. The NBCe2, with this stoichiometry, will mediate the efflux of HCO3- and Na+ from the cell into the cerebrospinal fluid at the apical membrane of the choroid plexus. © 2008 Elsevier Inc. All rights reserved.

KW - Cerebrospinal fluid

KW - Choroid plexus

KW - Electrogenic

KW - Epithelium

KW - Sodium-bicarbonate cotransporter

KW - Whole-cell path clamp

U2 - 10.1016/j.bbrc.2008.06.053

DO - 10.1016/j.bbrc.2008.06.053

M3 - Article

C2 - 18588858

SN - 1090-2104

VL - 373

SP - 550

EP - 554

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

NBCe2 exhibits a 3 HCO3-:1 Na+ stoichiometry in mouse choroid plexus epithelial cells

Abstract

Keywords

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