Nerve compound action current (NCAC) measurements and morphometric analysis in the proximal segment after nerve transection and repair in a rabbit model

Erik T. Walbeehm, E. B Michiel Dudok Van Heel, Paul D L Kuypers, Giorgio Terenghi, Steven E R Hovius

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In the evaluation of nerve regeneration using magneto-neurography (MNG), the proximal segment showed a reproducible decrease in peak-peak amplitude of the nerve compound action current's (NCAC) of 60%. To explain these changes, morphometry of myelinated axons in the proximal segment is compared to the MNG signals. A standardised nerve transection and reconstruction was performed in rabbits. NCACs were measured approximately 5 cm proximal to the lesion from operated and control nerves after 12 weeks. Histological samples were taken from the same area of the nerve where the NCACs were obtained. Results showed a decrease of the peak-peak amplitude of the NCAC of 57% compared to the control. Conduction velocity decreased 15% (not significant). Morphometry elicited a decrease in larger (10-15 μm) axons (284±134 vs 82±55) and an increase in smaller (2-5 μm) axons (1445±360 vs 1921±393). A strong correlation existed between the decrease in amplitude and the decrease in larger axons (0.85). Peak-peak amplitude varies approximately with the square of the diameter axon. Therefore, because peak-peak amplitude is mainly dependent on the larger-diameter axons, the decrease in peak-peak amplitude of the NCACs may be explained by a decrease in numbers of 10-15-μm axons.
    Original languageEnglish
    Pages (from-to)108-115
    Number of pages7
    JournalJournal of the Peripheral Nervous System
    Volume8
    Issue number2
    DOIs
    Publication statusPublished - Jun 2003

    Keywords

    • Histology
    • Magnetic neurography
    • Myelinated axon count
    • Nerve regeneration
    • Proximal segment

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