New Twists in Detecting mRNA Modification Dynamics

Ina Anreiter; Quoseena Mir; Jared T. Simpson; Sarath C. Janga; Matthias Soller

doi:10.1016/j.tibtech.2020.06.002

New Twists in Detecting mRNA Modification Dynamics

Ina Anreiter, Quoseena Mir, Jared T. Simpson, Sarath C. Janga, Matthias Soller^*

^*Corresponding author for this work

Division of Molecular & Cellular Function (L5)

Research output: Contribution to journal › Review article › peer-review

Abstract

Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances to increase confidence will be necessary. Single-molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations, and we summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

Original language	English
Pages (from-to)	72-89
Number of pages	18
Journal	Trends in Biotechnology
Volume	39
Issue number	1
Early online date	30 Jun 2020
DOIs	https://doi.org/10.1016/j.tibtech.2020.06.002
Publication status	Published - Jan 2021

Keywords

nanopore
mRNA modifications
m6A
m6Am
m5C
2′-O-ribose methylation

Access to Document

10.1016/j.tibtech.2020.06.002

Cite this

@article{fa7f55a5549e41b9bfaf67a203ec9e6b,

title = "New Twists in Detecting mRNA Modification Dynamics",

abstract = "Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances to increase confidence will be necessary. Single-molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations, and we summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.",

keywords = "nanopore, mRNA modifications, m6A, m6Am, m5C, 2′-O-ribose methylation",

author = "Ina Anreiter and Quoseena Mir and Simpson, \{Jared T.\} and Janga, \{Sarath C.\} and Matthias Soller",

year = "2021",

month = jan,

doi = "10.1016/j.tibtech.2020.06.002",

language = "English",

volume = "39",

pages = "72--89",

journal = "Trends in Biotechnology",

issn = "0167-7799",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - New Twists in Detecting mRNA Modification Dynamics

AU - Anreiter, Ina

AU - Mir, Quoseena

AU - Simpson, Jared T.

AU - Janga, Sarath C.

AU - Soller, Matthias

PY - 2021/1

Y1 - 2021/1

N2 - Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances to increase confidence will be necessary. Single-molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations, and we summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

AB - Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances to increase confidence will be necessary. Single-molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations, and we summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

KW - nanopore

KW - mRNA modifications

KW - m6A

KW - m6Am

KW - m5C

KW - 2′-O-ribose methylation

UR - https://www.scopus.com/pages/publications/85087220513

U2 - 10.1016/j.tibtech.2020.06.002

DO - 10.1016/j.tibtech.2020.06.002

M3 - Review article

SN - 0167-7799

VL - 39

SP - 72

EP - 89

JO - Trends in Biotechnology

JF - Trends in Biotechnology

IS - 1

ER -

New Twists in Detecting mRNA Modification Dynamics

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this