NF-X, a transcription factor implicated in MHC class II gene regulation

V Kouskoff, R M Mantovani, S M Candéias, A Dorn, A Staub, B Lisowska-Grospierre, C Griscelli, C O Benoist, D J Mathis

Research output: Contribution to journalArticlepeer-review

Abstract

The X box has been shown in several assay systems to be a critical element of MHC class II gene promoters. Several X box-binding activities have been discovered in nuclear extracts from a variety of cell lines. The critical question is: which of these are responsible for mediating X box function? This report provides a further characterization of NF-X, a highly specific X box-binding activity we described previously. The cell-type distribution, structural features, and binding site characteristics of NF-X are analyzed in detail, to facilitate comparison with other reported activities. Most importantly, the functional relevance of NF-X is assessed by scanning mutagenesis, and the results indicate that this complex is indeed involved in regulating MHC class II gene expression. With these data in mind, the relationship between NF-X and RF-X, an X box-binding activity reported to be absent in patients with severe combined immunodeficiency, is discussed.

Original languageEnglish
Pages (from-to)3197-204
Number of pages8
JournalJournal of immunology (Baltimore, Md. : 1950)
Volume146
Issue number9
Publication statusPublished - 1 May 1991

Keywords

  • Animals
  • Base Sequence
  • Cell Line
  • DNA-Binding Proteins
  • Gene Expression Regulation
  • HLA-D Antigens
  • Humans
  • Major Histocompatibility Complex
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins
  • Oligonucleotides
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
  • Transcription Factors
  • Transcription, Genetic
  • Journal Article
  • Research Support, Non-U.S. Gov't

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