Nitromethylene actions on in situ and expressed insect nicotinic acetylcholine receptors

C. A. Leech, P. Jewess, J. Marshall, D. B. Sattelle

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Single channel recordings from dissociated housefly (Musca domestica) neurons show that a novel type of nitromethylene insecticide, 2(nitromethylene)tetrahydro-1,3-thiazine (NMTHT) gates a channel, the conductance and open time histogram of which resemble those obtained when acetylcholine is the agonist. Injection into Xenopus oocytes of a locust (Schistocerca gregaria) α-subunit mRNA results in the expression of functional nicotinic receptors sensitive to NMTHT. Control oocytes injected with distilled water are insensitive to the same concentration of this compound. Thus NMTHT exhibits agonist actions at both in situ and expressed insect nicotinic receptors, and one site of action of this compound is on an insect nicotinic receptor α-subunit. © 1991.
    Original languageEnglish
    Pages (from-to)90-94
    Number of pages4
    JournalFEBS Letters
    Volume290
    Issue number1-2
    Publication statusPublished - 23 Sept 1991

    Keywords

    • Insect
    • Nicotinic receptor
    • Nitromethylene
    • Receptor subunit

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