NMR lipid profile of Agaricus bisporus

Pascale M A Bonzom, Anna Nicolaou, Mire Zloh, Wilfred Baldeo, William A. Gibbons

    Research output: Contribution to journalArticlepeer-review


    Lipids extracted from freeze dried and powdered cultivated Agaricus bisporus by the Bligh and Dwyer method, were subjected to 1D-proton and 2D- COSY NMR analysis. The diacylglycerophospholipids, mono-, di- and tri- glycerides, ether lipids, sphingolipids and steroidal lipids were studied qualitatively and quantitatively. Our findings suggested that (a) ethanolamines and cholines were the predominant diacylphospholipids, (b) sterols, mainly ergosterol, were present in relatively large quantities and (c) and phospholipid fatty acid composition consisted almost exclusively of linoleic acid. This type of detailed data on lipid composition was accurately and rapidly obtained in one step, without chemical modification of the sample. Additional information on four classes of lipid, including their fatty acid composition was obtained after separating the total lipid extract by NH2-aminopropyl Certify II Bond Elut solid phase chromatography and analysing the NMR spectra of each class of lipids. The results demonstrated the potential of the method for the study of plant metabolism, development and taxonomy.
    Original languageEnglish
    Pages (from-to)1311-1321
    Number of pages10
    Issue number8
    Publication statusPublished - 1 Apr 1999


    • Agaricus bisporus
    • Basidiomycetes
    • Lipids
    • Mushroom
    • NMR


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