No association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA

Kathryn L. Harrison; Philip A J Crosbie; Raymond M. Agius; Philip V. Barber; Mark Carus; Geoffrey P. Margison; Andrew C. Povey

doi:10.1016/j.mrfmmm.2006.03.005

No association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA

Kathryn L. Harrison, Philip A J Crosbie, Raymond M. Agius, Philip V. Barber, Mark Carus, Geoffrey P. Margison, Andrew C. Povey

Research output: Contribution to journal › Article › peer-review

Abstract

To examine associations between two different classes of DNA damage that can occur through endogenous processes or exogenous exposures such as smoking, N7-methyldeoxyguanosine (N7-MedG) and 8-oxodeoxyguanosine (8-oxodG) levels were measured in lymphocyte DNA from 22 bronchoscopy patients. 8-OxodG and N7-MedG was detected in 100% and 91% of samples, respectively with 8-oxodG levels being approx 20 times higher (mean 8.39 ± 3.57 8-oxodG/106dG versus 0.41 ± 0.33 N7-MedG/106 dG) which provides an indication of the relative importance of the agents that induce oxidative DNA damage or alkylation damage. The sources of these genotoxic lesions remain to be established but N7-MedG and 8-oxodG levels were not correlated (r2 <0.01) suggesting that there is no association between alkylating agent and reactive oxygen species exposure, their metabolism and/or the DNA repair processes that can remove this DNA damage. © 2006 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	125-130
Number of pages	5
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	600
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mrfmmm.2006.03.005
Publication status	Published - 30 Aug 2006

Keywords

8-Hydroxydeoxyguanosine
Alkylation
DNA damage
Lung cancer
N7-methyldeoxyguanosine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mrfmmm.2006.03.005

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@article{be29df4fa4d24797ae24c094b9f18a9f,

title = "No association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA",

abstract = "To examine associations between two different classes of DNA damage that can occur through endogenous processes or exogenous exposures such as smoking, N7-methyldeoxyguanosine (N7-MedG) and 8-oxodeoxyguanosine (8-oxodG) levels were measured in lymphocyte DNA from 22 bronchoscopy patients. 8-OxodG and N7-MedG was detected in 100% and 91% of samples, respectively with 8-oxodG levels being approx 20 times higher (mean 8.39 ± 3.57 8-oxodG/106dG versus 0.41 ± 0.33 N7-MedG/106 dG) which provides an indication of the relative importance of the agents that induce oxidative DNA damage or alkylation damage. The sources of these genotoxic lesions remain to be established but N7-MedG and 8-oxodG levels were not correlated (r2 <0.01) suggesting that there is no association between alkylating agent and reactive oxygen species exposure, their metabolism and/or the DNA repair processes that can remove this DNA damage. {\textcopyright} 2006 Elsevier B.V. All rights reserved.",

keywords = "8-Hydroxydeoxyguanosine, Alkylation, DNA damage, Lung cancer, N7-methyldeoxyguanosine",

author = "Harrison, {Kathryn L.} and Crosbie, {Philip A J} and Agius, {Raymond M.} and Barber, {Philip V.} and Mark Carus and Margison, {Geoffrey P.} and Povey, {Andrew C.}",

year = "2006",

month = aug,

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doi = "10.1016/j.mrfmmm.2006.03.005",

language = "English",

volume = "600",

pages = "125--130",

journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

issn = "0027-5107",

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TY - JOUR

T1 - No association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA

AU - Harrison, Kathryn L.

AU - Crosbie, Philip A J

AU - Agius, Raymond M.

AU - Barber, Philip V.

AU - Carus, Mark

AU - Margison, Geoffrey P.

AU - Povey, Andrew C.

PY - 2006/8/30

Y1 - 2006/8/30

N2 - To examine associations between two different classes of DNA damage that can occur through endogenous processes or exogenous exposures such as smoking, N7-methyldeoxyguanosine (N7-MedG) and 8-oxodeoxyguanosine (8-oxodG) levels were measured in lymphocyte DNA from 22 bronchoscopy patients. 8-OxodG and N7-MedG was detected in 100% and 91% of samples, respectively with 8-oxodG levels being approx 20 times higher (mean 8.39 ± 3.57 8-oxodG/106dG versus 0.41 ± 0.33 N7-MedG/106 dG) which provides an indication of the relative importance of the agents that induce oxidative DNA damage or alkylation damage. The sources of these genotoxic lesions remain to be established but N7-MedG and 8-oxodG levels were not correlated (r2 <0.01) suggesting that there is no association between alkylating agent and reactive oxygen species exposure, their metabolism and/or the DNA repair processes that can remove this DNA damage. © 2006 Elsevier B.V. All rights reserved.

AB - To examine associations between two different classes of DNA damage that can occur through endogenous processes or exogenous exposures such as smoking, N7-methyldeoxyguanosine (N7-MedG) and 8-oxodeoxyguanosine (8-oxodG) levels were measured in lymphocyte DNA from 22 bronchoscopy patients. 8-OxodG and N7-MedG was detected in 100% and 91% of samples, respectively with 8-oxodG levels being approx 20 times higher (mean 8.39 ± 3.57 8-oxodG/106dG versus 0.41 ± 0.33 N7-MedG/106 dG) which provides an indication of the relative importance of the agents that induce oxidative DNA damage or alkylation damage. The sources of these genotoxic lesions remain to be established but N7-MedG and 8-oxodG levels were not correlated (r2 <0.01) suggesting that there is no association between alkylating agent and reactive oxygen species exposure, their metabolism and/or the DNA repair processes that can remove this DNA damage. © 2006 Elsevier B.V. All rights reserved.

KW - 8-Hydroxydeoxyguanosine

KW - Alkylation

KW - DNA damage

KW - Lung cancer

KW - N7-methyldeoxyguanosine

U2 - 10.1016/j.mrfmmm.2006.03.005

DO - 10.1016/j.mrfmmm.2006.03.005

M3 - Article

C2 - 16765387

SN - 0027-5107

VL - 600

SP - 125

EP - 130

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

IS - 1-2

ER -

No association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA

Abstract

Keywords

UN SDGs

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