Non-covalent protein-based adhesives for transparent substrates –  bovine serum albumin vs. recombinant spider silk

Aled Roberts; William Finnigan; Paul Kelly; Matthew Faulkner; Rainer Breitling; Eriko Takano; Nigel Scrutton; Jonny Blaker; Sam Hay

doi:10.1016/j.mtbio.2020.100068

Non-covalent protein-based adhesives for transparent substrates – bovine serum albumin vs. recombinant spider silk

Aled Roberts, William Finnigan, Paul Kelly, Matthew Faulkner, Rainer Breitling, Eriko Takano, Nigel Scrutton, Jonny Blaker, Sam Hay

Research output: Contribution to journal › Article › peer-review

Abstract

Protein-based adhesives could have several advantages over petroleum-derived alternatives, including substantially lower toxicity, smaller environmental footprint and renewable sourcing. Here, we report that non-covalently crosslinked bovine serum albumin (BSA) and recombinant spider silk proteins have high adhesive strength on glass (8.53 and 6.28 MPa, respectively) and other transparent substrates. Moreover, the adhesives have high visible transparency and showed no apparent degradation over a period of several months. The mechanism of adhesion was investigated and primarily attributed to dehydration-induced reorganisation of protein secondary structure, resulting in the supramolecular association of β-sheets into a densely hydrogen-bonded network.

Original language	English
Pages (from-to)	100068
Journal	Materials Today Bio
Volume	7
DOIs	https://doi.org/10.1016/j.mtbio.2020.100068
Publication status	Published - 10 Jul 2020

Access to Document

10.1016/j.mtbio.2020.100068Licence: CC BY

Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals
Scrutton, N. (PI), Azapagic, A. (CoI), Balmer, A. (CoI), Barran, P. (CoI), Breitling, R. (CoI), Delneri, D. (CoI), Dixon, N. (CoI), Faulon, J.-L. (CoI), Flitsch, S. (CoI), Goble, C. (CoI), Goodacre, R. (CoI), Hay, S. (CoI), Kell, D. (CoI), Leys, D. (CoI), Lloyd, J. (CoI), Lockyer, N. (CoI), Martin, P. (CoI), Micklefield, J. (CoI), Munro, A. (CoI), Pedrosa Mendes, P. (CoI), Randles, S. (CoI), Salehi Yazdi, F. (CoI), Shapira, P. (CoI), Takano, E. (CoI), Turner, N. (CoI) & Winterburn, J. (CoI)
14/11/14 → 13/05/20
Project: Research

Cite this

@article{47919b2e06bf4cc2bb1042565df79e9b,

title = "Non-covalent protein-based adhesives for transparent substrates – bovine serum albumin vs. recombinant spider silk",

abstract = "Protein-based adhesives could have several advantages over petroleum-derived alternatives, including substantially lower toxicity, smaller environmental footprint and renewable sourcing. Here, we report that non-covalently crosslinked bovine serum albumin (BSA) and recombinant spider silk proteins have high adhesive strength on glass (8.53 and 6.28 MPa, respectively) and other transparent substrates. Moreover, the adhesives have high visible transparency and showed no apparent degradation over a period of several months. The mechanism of adhesion was investigated and primarily attributed to dehydration-induced reorganisation of protein secondary structure, resulting in the supramolecular association of β-sheets into a densely hydrogen-bonded network.",

author = "Aled Roberts and William Finnigan and Paul Kelly and Matthew Faulkner and Rainer Breitling and Eriko Takano and Nigel Scrutton and Jonny Blaker and Sam Hay",

year = "2020",

month = jul,

day = "10",

doi = "10.1016/j.mtbio.2020.100068",

language = "English",

volume = "7",

pages = "100068",

journal = "Materials Today Bio",

issn = "2590-0064",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Non-covalent protein-based adhesives for transparent substrates – bovine serum albumin vs. recombinant spider silk

AU - Roberts, Aled

AU - Finnigan, William

AU - Kelly, Paul

AU - Faulkner, Matthew

AU - Breitling, Rainer

AU - Takano, Eriko

AU - Scrutton, Nigel

AU - Blaker, Jonny

AU - Hay, Sam

PY - 2020/7/10

Y1 - 2020/7/10

N2 - Protein-based adhesives could have several advantages over petroleum-derived alternatives, including substantially lower toxicity, smaller environmental footprint and renewable sourcing. Here, we report that non-covalently crosslinked bovine serum albumin (BSA) and recombinant spider silk proteins have high adhesive strength on glass (8.53 and 6.28 MPa, respectively) and other transparent substrates. Moreover, the adhesives have high visible transparency and showed no apparent degradation over a period of several months. The mechanism of adhesion was investigated and primarily attributed to dehydration-induced reorganisation of protein secondary structure, resulting in the supramolecular association of β-sheets into a densely hydrogen-bonded network.

AB - Protein-based adhesives could have several advantages over petroleum-derived alternatives, including substantially lower toxicity, smaller environmental footprint and renewable sourcing. Here, we report that non-covalently crosslinked bovine serum albumin (BSA) and recombinant spider silk proteins have high adhesive strength on glass (8.53 and 6.28 MPa, respectively) and other transparent substrates. Moreover, the adhesives have high visible transparency and showed no apparent degradation over a period of several months. The mechanism of adhesion was investigated and primarily attributed to dehydration-induced reorganisation of protein secondary structure, resulting in the supramolecular association of β-sheets into a densely hydrogen-bonded network.

U2 - 10.1016/j.mtbio.2020.100068

DO - 10.1016/j.mtbio.2020.100068

M3 - Article

SN - 2590-0064

VL - 7

SP - 100068

JO - Materials Today Bio

JF - Materials Today Bio

ER -

Non-covalent protein-based adhesives for transparent substrates – bovine serum albumin vs. recombinant spider silk

Abstract

Access to Document

Fingerprint

Projects

Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals

Cite this