Novel Cell Line Selectively Expressing Neuropeptide Y-Y2 Receptors

Martin M. Höfliger, Gloria López Castejón, Wieland Kiess, Annette G. Beck Sickinger

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Neuropeptide Y (NPY) recognition by the human neuroblastoma cell lines SiMa, Kelly, SH-SY5Y, CHP-234, and MHH-NB-11 was analyzed in radioactive binding assays using tritiated NPY. For the cell lines CHP-234 and MHH-NB-11 binding of [3H]propionyl-NPY was observed with Kd-values of 0.64±0.07 nM and 0.53± 0.12 nM, respectively, determined by saturation analysis with non-linear regression. The receptor subtype was determined by competition analysis using the subtype selective NPY analogues [Leu31, Pro34]-NPY (NPY-Y1, NPY-Y5), [Ahx5-24]-NPY (NPY-Y2), [Ala31, Aib 32]-NPY (NPY-Y5), NPY [3-36] (NPY-Y2, NPY-Y5), and NPY [13-36] (NPY-Y2). Both cell lines, CHP-234 and MHH-NB-11, the latter one being characterized for NPY receptors for the first time, showed exclusive expression of NPY-Y2 receptors. In both cell lines binding of NPY induced signal transduction, which was monitored as reduction of forskolin-induced cAMP production in an ELISA.
    Original languageEnglish
    Pages (from-to)351-360
    Number of pages9
    JournalJournal of Receptors and Signal Transduction
    Volume23
    Issue number4
    DOIs
    Publication statusPublished - 2003

    Keywords

    • Binding
    • Cell line
    • Neuropeptide Y
    • Signal transduction
    • Y2 receptor

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