Novel lethal mouse mutants produced in balancer chromosome screens

Kathryn E. Hentges, Hisashi Nakamura, Yasuhide Furuta, Yuejin Yu, Debrah M. Thompson, William O'Brien, Allan Bradley, Monica J. Justice

    Research output: Contribution to journalArticlepeer-review


    Mutagenesis screens are a valuable method to identify genes that are required for normal development. Previous mouse mutagenesis screens for lethal mutations were targeted at specific time points or for developmental processes. Here we present the results of lethal mutant isolation from two mutagenesis screens that use balancer chromosomes. One screen was localized to mouse chromosome 4, between the STS markers D4Mit281 and D4Mit51. The second screen covered the region between Trp53 and Wnt3 on mouse chromosome 11. These screens identified all lethal mutations in the balancer regions, without bias towards any phenotype or stage of death. We have isolated 19 lethal lines on mouse chromosome 4, and 59 lethal lines on chromosome 11, many of which are distinct from previous mutants that map to these regions of the genome. We have characterized the mutant lines to determine the time of death, and performed a pair-wise complementation cross to determine if the mutations are allelic. Our data suggest that the majority of mouse lethal mutations die during mid-gestation, after uterine implantation, with a variety of defects in gastrulation, heart, neural tube, vascular, or placental development. This initial group of mutants provides a functional annotation of mouse chromosomes 4 and 11, and indicates that many novel developmental phenotypes can be quickly isolated in defined genomic intervals through balancer chromosome mutagenesis screens. © 2005 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)653-665
    Number of pages12
    JournalGene Expression Patterns
    Issue number6
    Publication statusPublished - Aug 2006


    • Balancer chromosome
    • Cardiovascular development
    • Embryonic development
    • Gastrulation defect
    • Lethal mutation
    • Mutagenesis screen
    • Peri-natal lethal
    • Post-natal lethal


    Dive into the research topics of 'Novel lethal mouse mutants produced in balancer chromosome screens'. Together they form a unique fingerprint.

    Cite this