Nuclear autoantigen p330d/CENP-F: A marker for cell proliferation in human malignancies

Goran Landberg; Göran Landberg; Martin Erlanson; Göran Roos; Eng M. Tan; Carlos A. Casiano

doi:10.1002/(SICI)1097-0320(19960901)25:1<90::AID-CYTO10>3.0.CO;2-P

Nuclear autoantigen p330d/CENP-F: A marker for cell proliferation in human malignancies

Goran Landberg, Göran Landberg, Martin Erlanson, Göran Roos, Eng M. Tan, Carlos A. Casiano

Research output: Contribution to journal › Article › peer-review

Abstract

p330d, also known as CENP-F, is a newly characterized cell cycle specific nuclear autoantigen which is associated both with the centromeres and the nuclear matrix. It is expressed in low amounts in G0/G1-cells and accumulates in the nuclear matrix during S-phase with a maximum expression in G2/M-cells. In the present study we have investigated if p330d/CENP-F could be used as a marker for proliferation in different human malignancies. A flow cytometric method was developed by which p330d/CENP-F expression and DNA-content could be assessed on hematopoietic and solid tumors. Twenty-four different human hematopoietic malignancies, 12 breast cancers, and several cell lines were analyzed and the number of p330d/CENP-F positive cells and the S-phase fraction were determined. The percentage of p330d/CENP-F positive cells correlated with the fraction of S-phase cells in all human malignancies tested. Various cell lines revealed a similar cell cycle specific distribution. The association of p330d/CENP-F with the nuclear matrix facilitated the now cytometric analysis of this protein due to its resistance to different preparation and fixation procedures. In summary, p330d/ CENP-F seems to be a potentially valuable proliferation marker which can be applied to different tumors.

Original language	English
Pages (from-to)	90-98
Number of pages	8
Journal	Cytometry
Volume	25
Issue number	1
DOIs	https://doi.org/10.1002/(SICI)1097-0320(19960901)25:1<90::AID-CYTO10>3.0.CO;2-P
Publication status	Published - 1996

Keywords

Autoantibodies
Cell cycle
Centromere
Flow cytometry
Nuclear matrix
Proliferation

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/(SICI)1097-0320(19960901)25:1<90::AID-CYTO10>3.0.CO;2-P

Cite this

@article{7908e74a32e94eddaaad7c5cc49810b9,

title = "Nuclear autoantigen p330d/CENP-F: A marker for cell proliferation in human malignancies",

abstract = "p330d, also known as CENP-F, is a newly characterized cell cycle specific nuclear autoantigen which is associated both with the centromeres and the nuclear matrix. It is expressed in low amounts in G0/G1-cells and accumulates in the nuclear matrix during S-phase with a maximum expression in G2/M-cells. In the present study we have investigated if p330d/CENP-F could be used as a marker for proliferation in different human malignancies. A flow cytometric method was developed by which p330d/CENP-F expression and DNA-content could be assessed on hematopoietic and solid tumors. Twenty-four different human hematopoietic malignancies, 12 breast cancers, and several cell lines were analyzed and the number of p330d/CENP-F positive cells and the S-phase fraction were determined. The percentage of p330d/CENP-F positive cells correlated with the fraction of S-phase cells in all human malignancies tested. Various cell lines revealed a similar cell cycle specific distribution. The association of p330d/CENP-F with the nuclear matrix facilitated the now cytometric analysis of this protein due to its resistance to different preparation and fixation procedures. In summary, p330d/ CENP-F seems to be a potentially valuable proliferation marker which can be applied to different tumors.",

keywords = "Autoantibodies, Cell cycle, Centromere, Flow cytometry, Nuclear matrix, Proliferation",

author = "Goran Landberg and G{\"o}ran Landberg and Martin Erlanson and G{\"o}ran Roos and Tan, {Eng M.} and Casiano, {Carlos A.}",

note = "CA-56956, NCI NIH HHS, United States",

year = "1996",

doi = "10.1002/(SICI)1097-0320(19960901)25:1<90::AID-CYTO10>3.0.CO;2-P",

language = "English",

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pages = "90--98",

journal = "Cytometry",

publisher = "John Wiley & Sons Ltd",

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TY - JOUR

T1 - Nuclear autoantigen p330d/CENP-F: A marker for cell proliferation in human malignancies

AU - Landberg, Goran

AU - Landberg, Göran

AU - Erlanson, Martin

AU - Roos, Göran

AU - Tan, Eng M.

AU - Casiano, Carlos A.

N1 - CA-56956, NCI NIH HHS, United States

PY - 1996

Y1 - 1996

N2 - p330d, also known as CENP-F, is a newly characterized cell cycle specific nuclear autoantigen which is associated both with the centromeres and the nuclear matrix. It is expressed in low amounts in G0/G1-cells and accumulates in the nuclear matrix during S-phase with a maximum expression in G2/M-cells. In the present study we have investigated if p330d/CENP-F could be used as a marker for proliferation in different human malignancies. A flow cytometric method was developed by which p330d/CENP-F expression and DNA-content could be assessed on hematopoietic and solid tumors. Twenty-four different human hematopoietic malignancies, 12 breast cancers, and several cell lines were analyzed and the number of p330d/CENP-F positive cells and the S-phase fraction were determined. The percentage of p330d/CENP-F positive cells correlated with the fraction of S-phase cells in all human malignancies tested. Various cell lines revealed a similar cell cycle specific distribution. The association of p330d/CENP-F with the nuclear matrix facilitated the now cytometric analysis of this protein due to its resistance to different preparation and fixation procedures. In summary, p330d/ CENP-F seems to be a potentially valuable proliferation marker which can be applied to different tumors.

AB - p330d, also known as CENP-F, is a newly characterized cell cycle specific nuclear autoantigen which is associated both with the centromeres and the nuclear matrix. It is expressed in low amounts in G0/G1-cells and accumulates in the nuclear matrix during S-phase with a maximum expression in G2/M-cells. In the present study we have investigated if p330d/CENP-F could be used as a marker for proliferation in different human malignancies. A flow cytometric method was developed by which p330d/CENP-F expression and DNA-content could be assessed on hematopoietic and solid tumors. Twenty-four different human hematopoietic malignancies, 12 breast cancers, and several cell lines were analyzed and the number of p330d/CENP-F positive cells and the S-phase fraction were determined. The percentage of p330d/CENP-F positive cells correlated with the fraction of S-phase cells in all human malignancies tested. Various cell lines revealed a similar cell cycle specific distribution. The association of p330d/CENP-F with the nuclear matrix facilitated the now cytometric analysis of this protein due to its resistance to different preparation and fixation procedures. In summary, p330d/ CENP-F seems to be a potentially valuable proliferation marker which can be applied to different tumors.

KW - Autoantibodies

KW - Cell cycle

KW - Centromere

KW - Flow cytometry

KW - Nuclear matrix

KW - Proliferation

U2 - 10.1002/(SICI)1097-0320(19960901)25:1<90::AID-CYTO10>3.0.CO;2-P

DO - 10.1002/(SICI)1097-0320(19960901)25:1<90::AID-CYTO10>3.0.CO;2-P

M3 - Article

C2 - 8875058

VL - 25

SP - 90

EP - 98

JO - Cytometry

JF - Cytometry

IS - 1

ER -

Nuclear autoantigen p330d/CENP-F: A marker for cell proliferation in human malignancies

Abstract

Keywords

UN SDGs

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