Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading

Hamish Gilbert, Venkatesh Mallikarjun, Oana Dobre, Mark Jackson, Robert Pedley, Andrew Gilmore, Stephen Richardson, Joe Swift

Research output: Contribution to journalArticlepeer-review

Abstract

Studies of cellular mechano-signaling have often utilised static models that do not fully replicate the dynamics of living tissues. Here, we examine the time-dependent response of primary human mesenchymal stem cells (hMSCs) to cyclic tensile strain (CTS). At low-intensity strain (1 hour, 4% CTS at 1 Hz) cell characteristics mimic responses to increased substrate stiffness. As the strain regime is intensified (frequency increased to 5 Hz), we characterise rapid establishment of a broad, structured and reversible protein-level response, even as transcription is apparently downregulated. Protein abundance is quantified coincident with changes to protein conformation and post translational modification (PTM). Furthermore, we characterise changes to the linker of nucleoskeleton and cytoskeleton (LINC) complex that bridges the nuclear envelope, and specifically to levels and PTMs of Sad1/UNC-84 (SUN) domain-containing protein 2 (SUN2). The result of this regulation is to decouple mechano-transmission between the cytoskeleton and the nucleus, thus conferring protection to chromatin.
Original languageEnglish
Article number4149
JournalNature Communications
Volume10
DOIs
Publication statusPublished - 12 Sept 2019

Fingerprint

Dive into the research topics of 'Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading'. Together they form a unique fingerprint.

Cite this