Abstract
Drosophila Ceramide Synthase (CerS) Schlank regulates both ceramide synthesis and fat metabolism. Schlank contains a catalytic lag1p motif and, like many CerS in other species, a homeodomain of unknown function. Here, we show that the Drosophila CerS Schlank is imported into the nucleus and requires two nuclear localization signals (NLSs) within its homeodomain and functional Importin-β import machinery. Expression of Schlank variants containing the homeodomain without functional lag1p motif rescued the fat metabolism phenotype of schlank mutants whereas a variant with a mutated NLS site did not rescue. Thus, the homeodomain of Schlank is involved in the regulation of lipid metabolism independent of the catalytic lag1p motif.
| Original language | English |
|---|---|
| Pages (from-to) | 971-81 |
| Number of pages | 11 |
| Journal | FEBS Letters |
| Volume | 590 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Apr 2016 |
Keywords
- Active Transport, Cell Nucleus
- Amino Acid Motifs
- Amino Acid Substitution
- Animals
- Animals, Genetically Modified
- Catalytic Domain
- Cell Line
- Cell Nucleus
- Drosophila Proteins
- Drosophila melanogaster
- Fat Body
- Green Fluorescent Proteins
- Homeodomain Proteins
- Lipid Metabolism
- Mutation
- Nuclear Localization Signals
- Peptide Fragments
- Protein Interaction Domains and Motifs
- RNA Interference
- Recombinant Fusion Proteins
- Sphingosine N-Acyltransferase
- beta Karyopherins
- Journal Article
- Research Support, Non-U.S. Gov't