TY - JOUR
T1 - Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage
AU - Grzechnik, Pawel
AU - Szczepaniak, Sylwia A.
AU - Dhir, Somdutta
AU - Pastucha, Anna
AU - Parslow, Hannah
AU - Matuszek, Zaneta
AU - Mischo, Hannah E.
AU - Kufel, Joanna
AU - Proudfoot, Nicholas J.
N1 - Funding Information:
We thank A. Hinnebusch for CBP20-myc and CBP80-myc, G. Chanfreau for rnt1E320K and T.H. Jensen for ceg1-63 strains. The JK group was supported by the Polish Ministry of Science and Higher Education (N N301 065740 to J.K.), Polish-Swiss Research Programme (PSPB-183/2010 to JK) National Science Centre (UMO-2011/01/N/NZ1/04344 to SS) and EU Social Fund (UDAPOKL.04.01.01-00-072/09-00). The JK group used CePT infrastructure financed by the EU Regional Development Fund (Innovative economy 2007-13, POIG.02.02.00-14-024/08-00). The PG group is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (200473/Z/16/Z). The NJP laboratory was supported by a Wellcome Trust Programme Grant (091805/Z/10/Z) and European Research Council Advanced Grant (339270). We thank the High-Throughput Genomics Group at the Wellcome Trust Centre for Human Genetics (funded by Wellcome Trust grant reference 203141/Z/16/Z) for the generation of the Sequencing data.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Small nucleolar RNA (snoRNA) are conserved and essential non-coding RNA that are transcribed by RNA Polymerase II (Pol II). Two snoRNA classes, formerly distinguished by their structure and ribonucleoprotein composition, act as guide RNA to target RNA such as ribosomal RNA, and thereby introduce specific modifications. We have studied the 5? end processing of individually transcribed snoRNA in S. cerevisiae to define their role in snoRNA biogenesis and functionality. Here we show that pre-snoRNA processing by the endonuclease Rnt1 occurs co-transcriptionally with removal of the m7G cap facilitating the formation of box C/D snoRNA. Failure of this process causes aberrant 3? end processing and mislocalization of snoRNA to the cytoplasm. Consequently, Rnt1-dependent 5? end processing of box C/D snoRNA is critical for snoRNA-dependent methylation of ribosomal RNA. Our results reveal that the 5 end processing of box C/D snoRNA defines their distinct pathway of maturation.
AB - Small nucleolar RNA (snoRNA) are conserved and essential non-coding RNA that are transcribed by RNA Polymerase II (Pol II). Two snoRNA classes, formerly distinguished by their structure and ribonucleoprotein composition, act as guide RNA to target RNA such as ribosomal RNA, and thereby introduce specific modifications. We have studied the 5? end processing of individually transcribed snoRNA in S. cerevisiae to define their role in snoRNA biogenesis and functionality. Here we show that pre-snoRNA processing by the endonuclease Rnt1 occurs co-transcriptionally with removal of the m7G cap facilitating the formation of box C/D snoRNA. Failure of this process causes aberrant 3? end processing and mislocalization of snoRNA to the cytoplasm. Consequently, Rnt1-dependent 5? end processing of box C/D snoRNA is critical for snoRNA-dependent methylation of ribosomal RNA. Our results reveal that the 5 end processing of box C/D snoRNA defines their distinct pathway of maturation.
UR - http://www.scopus.com/inward/record.url?scp=85046417238&partnerID=8YFLogxK
U2 - 10.1038/s41467-018-04094-y
DO - 10.1038/s41467-018-04094-y
M3 - Article
C2 - 29725044
AN - SCOPUS:85046417238
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1783
ER -