Nuclear magnetic resonance imaging measurements of water diffusion in the perfused hippocampal slice during N-methyl-D-aspartate-induced excitotoxicity

David Buckley, J. D. Bui, D. L. Buckley, M. I. Phillips, S. J. Blackband

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Significant changes in the apparent diffusion coefficient of water are observed in nuclear magnetic resonance images of patients with acute ischemic stroke. However, the underlying mechanisms of these apparent diffusion coefficient changes are still unresolved. To analyse possible mechanisms, this study applies nuclear magnetic resonance imaging on a 14.1Tesla narrow-bore magnet to quantitatively study water diffusion in individually perfused brain slices following exposure to N-methyl-D-aspartate excitotoxicity. The results indicate that brain slices have at least two distinct diffusing water compartments with apparent diffusion coefficients of 0.96±0.10x10-3mm2/s and 0.06±0.01x10-3mm2/s. When excitotoxicity was induced with N-methyl-D-aspartate, there was a significant decrease in the fraction of the fast diffusing water component in the slices (P
    Original languageEnglish
    Pages (from-to)487-490
    Number of pages3
    JournalNeuroscience
    Volume93
    Issue number2
    Publication statusPublished - Jul 1999

    Keywords

    • Brain slice
    • Cell volume
    • Ischemia
    • NMDA excitotoxicity
    • NMR imaging
    • Water diffusion

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