Nucleotide sequence of a gene from phanerochaete chrysosporium that shows homology to the faca gene of aspergillus nidulans

Paul R J Birch; Paul F G Sims; Paul Broda

doi:10.3109/10425179209030965

Nucleotide sequence of a gene from phanerochaete chrysosporium that shows homology to the faca gene of aspergillus nidulans

Paul R J Birch, Paul F G Sims, Paul Broda

Research output: Contribution to journal › Article › peer-review

Abstract

Heterologous hybridisation was used to isolate a genomic DNA sequence from Phanerochaete chrysosporium using the facA (acetyl CoA synthetase) gene from Aspergillus nidulans as a probe. The cloned sequence hybridises to a 2.2 kb transcript in poly(A)+ RNA prepared from mycelium grown on acetate as the sole carbon source. Comparison of the DNA sequence obtained with those of the A. nidulans facA and N. crassa acu5 genes reveals an ORF that appears to be interrupted by five typical fungal introns. Two possible candidates for the translation initiation codon were observed. Homology with the facA and acu5 genes is revealed after the second ATC codon. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Original language	English
Pages (from-to)	319-323
Number of pages	4
Journal	Mitochondrial DNA
Volume	2
Issue number	5
DOIs	https://doi.org/10.3109/10425179209030965
Publication status	Published - 1992

Keywords

Acetate utilising enzyme
Heterologous hybridization
Introns
Nucleotide sequence

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title = "Nucleotide sequence of a gene from phanerochaete chrysosporium that shows homology to the faca gene of aspergillus nidulans",

abstract = "Heterologous hybridisation was used to isolate a genomic DNA sequence from Phanerochaete chrysosporium using the facA (acetyl CoA synthetase) gene from Aspergillus nidulans as a probe. The cloned sequence hybridises to a 2.2 kb transcript in poly(A)+ RNA prepared from mycelium grown on acetate as the sole carbon source. Comparison of the DNA sequence obtained with those of the A. nidulans facA and N. crassa acu5 genes reveals an ORF that appears to be interrupted by five typical fungal introns. Two possible candidates for the translation initiation codon were observed. Homology with the facA and acu5 genes is revealed after the second ATC codon. {\textcopyright} 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.",

keywords = "Acetate utilising enzyme, Heterologous hybridization, Introns, Nucleotide sequence",

author = "Birch, {Paul R J} and Sims, {Paul F G} and Paul Broda",

year = "1992",

doi = "10.3109/10425179209030965",

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T1 - Nucleotide sequence of a gene from phanerochaete chrysosporium that shows homology to the faca gene of aspergillus nidulans

AU - Birch, Paul R J

AU - Sims, Paul F G

AU - Broda, Paul

PY - 1992

Y1 - 1992

N2 - Heterologous hybridisation was used to isolate a genomic DNA sequence from Phanerochaete chrysosporium using the facA (acetyl CoA synthetase) gene from Aspergillus nidulans as a probe. The cloned sequence hybridises to a 2.2 kb transcript in poly(A)+ RNA prepared from mycelium grown on acetate as the sole carbon source. Comparison of the DNA sequence obtained with those of the A. nidulans facA and N. crassa acu5 genes reveals an ORF that appears to be interrupted by five typical fungal introns. Two possible candidates for the translation initiation codon were observed. Homology with the facA and acu5 genes is revealed after the second ATC codon. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

AB - Heterologous hybridisation was used to isolate a genomic DNA sequence from Phanerochaete chrysosporium using the facA (acetyl CoA synthetase) gene from Aspergillus nidulans as a probe. The cloned sequence hybridises to a 2.2 kb transcript in poly(A)+ RNA prepared from mycelium grown on acetate as the sole carbon source. Comparison of the DNA sequence obtained with those of the A. nidulans facA and N. crassa acu5 genes reveals an ORF that appears to be interrupted by five typical fungal introns. Two possible candidates for the translation initiation codon were observed. Homology with the facA and acu5 genes is revealed after the second ATC codon. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

KW - Acetate utilising enzyme

KW - Heterologous hybridization

KW - Introns

KW - Nucleotide sequence

U2 - 10.3109/10425179209030965

DO - 10.3109/10425179209030965

M3 - Article

SN - 1940-1744

VL - 2

SP - 319

EP - 323

JO - Mitochondrial DNA

JF - Mitochondrial DNA

IS - 5

ER -

Nucleotide sequence of a gene from phanerochaete chrysosporium that shows homology to the faca gene of aspergillus nidulans

Abstract

Keywords

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