Observational study on variability between biobanks in the estimation of DNA concentration

Jay Brown; Alexander N. Donev; Charalampos Aslanidis; Pippa Bracegirdle; Katherine P. Dixon; Manuela Foedinger; Rhian Gwilliam; Matthew Hardy; Thomas Illig; Xiayi Ke; Dagni Krinka; Camilla Lagerberg; Päivi Laiho; David H. Lewis; Wendy McArdle; Simon Patton; Susan M. Ring; Gerd Schmitz; Helen Stevens; Gunnel Tybring; H. Erich Wichmann; William Er Ollier; Martin A. Yuille

doi:10.1186/1756-0500-2-208

Observational study on variability between biobanks in the estimation of DNA concentration

Jay Brown, Alexander N. Donev, Charalampos Aslanidis, Pippa Bracegirdle, Katherine P. Dixon, Manuela Foedinger, Rhian Gwilliam, Matthew Hardy, Thomas Illig, Xiayi Ke, Dagni Krinka, Camilla Lagerberg, Päivi Laiho, David H. Lewis, Wendy McArdle, Simon Patton, Susan M. Ring, Gerd Schmitz, Helen Stevens, Gunnel TybringH. Erich Wichmann, William Er Ollier, Martin A. Yuille

Research output: Contribution to journal › Article › peer-review

Abstract

Background. There is little confidence in the consistency of estimation of DNA concentrations when samples move between laboratories. Evidence on this consistency is largely anecdotal. Therefore there is a need first to measure this consistency among different laboratories and then identify and implement remedies. A pilot experiment to test logistics and provide initial data on consistency was therefore conceived. Methods. DNA aliquots at nominal concentrations between 10 and 300 ng/l were dispensed into the wells of 96-well plates by one participant - the coordinating centre. Participants estimated the concentration in each well and returned estimates to the coordinating centre. Results. Considerable overall variability was observed among estimates. There were statistically significant differences between participants' measurements and between fluorescence emission and absorption spectroscopy. Conclusion. Anecdotal evidence of variability in DNA concentration estimation has been substantiated. Reduction in variability between participants will require the identification of major sources of variation, specification of effective remedies and their implementation. © 2009 Yuille et al.

Original language	English
Article number	208
Journal	BMC Research Notes
Volume	2
DOIs	https://doi.org/10.1186/1756-0500-2-208
Publication status	Published - 2009

Access to Document

10.1186/1756-0500-2-208

Cite this

Brown, J., Donev, A. N., Aslanidis, C., Bracegirdle, P., Dixon, K. P., Foedinger, M., Gwilliam, R., Hardy, M., Illig, T., Ke, X., Krinka, D., Lagerberg, C., Laiho, P., Lewis, D. H., McArdle, W., Patton, S., Ring, S. M., Schmitz, G., Stevens, H., ... Yuille, M. A. (2009). Observational study on variability between biobanks in the estimation of DNA concentration. BMC Research Notes, 2, Article 208. https://doi.org/10.1186/1756-0500-2-208

@article{4c1cdd4164c44a729c07234be7e46bd7,

title = "Observational study on variability between biobanks in the estimation of DNA concentration",

abstract = "Background. There is little confidence in the consistency of estimation of DNA concentrations when samples move between laboratories. Evidence on this consistency is largely anecdotal. Therefore there is a need first to measure this consistency among different laboratories and then identify and implement remedies. A pilot experiment to test logistics and provide initial data on consistency was therefore conceived. Methods. DNA aliquots at nominal concentrations between 10 and 300 ng/l were dispensed into the wells of 96-well plates by one participant - the coordinating centre. Participants estimated the concentration in each well and returned estimates to the coordinating centre. Results. Considerable overall variability was observed among estimates. There were statistically significant differences between participants' measurements and between fluorescence emission and absorption spectroscopy. Conclusion. Anecdotal evidence of variability in DNA concentration estimation has been substantiated. Reduction in variability between participants will require the identification of major sources of variation, specification of effective remedies and their implementation. {\textcopyright} 2009 Yuille et al.",

author = "Jay Brown and Donev, {Alexander N.} and Charalampos Aslanidis and Pippa Bracegirdle and Dixon, {Katherine P.} and Manuela Foedinger and Rhian Gwilliam and Matthew Hardy and Thomas Illig and Xiayi Ke and Dagni Krinka and Camilla Lagerberg and P{\"a}ivi Laiho and Lewis, {David H.} and Wendy McArdle and Simon Patton and Ring, {Susan M.} and Gerd Schmitz and Helen Stevens and Gunnel Tybring and Wichmann, {H. Erich} and Ollier, {William Er} and Yuille, {Martin A.}",

year = "2009",

doi = "10.1186/1756-0500-2-208",

language = "English",

volume = "2",

journal = "BMC Research Notes",

issn = "1756-0500",

publisher = "Springer Nature",

}

Brown, J, Donev, AN, Aslanidis, C, Bracegirdle, P, Dixon, KP, Foedinger, M, Gwilliam, R, Hardy, M, Illig, T, Ke, X, Krinka, D, Lagerberg, C, Laiho, P, Lewis, DH, McArdle, W, Patton, S, Ring, SM, Schmitz, G, Stevens, H, Tybring, G, Wichmann, HE, Ollier, WE & Yuille, MA 2009, 'Observational study on variability between biobanks in the estimation of DNA concentration', BMC Research Notes, vol. 2, 208. https://doi.org/10.1186/1756-0500-2-208

TY - JOUR

T1 - Observational study on variability between biobanks in the estimation of DNA concentration

AU - Brown, Jay

AU - Donev, Alexander N.

AU - Aslanidis, Charalampos

AU - Bracegirdle, Pippa

AU - Dixon, Katherine P.

AU - Foedinger, Manuela

AU - Gwilliam, Rhian

AU - Hardy, Matthew

AU - Illig, Thomas

AU - Ke, Xiayi

AU - Krinka, Dagni

AU - Lagerberg, Camilla

AU - Laiho, Päivi

AU - Lewis, David H.

AU - McArdle, Wendy

AU - Patton, Simon

AU - Ring, Susan M.

AU - Schmitz, Gerd

AU - Stevens, Helen

AU - Tybring, Gunnel

AU - Wichmann, H. Erich

AU - Ollier, William Er

AU - Yuille, Martin A.

PY - 2009

Y1 - 2009

N2 - Background. There is little confidence in the consistency of estimation of DNA concentrations when samples move between laboratories. Evidence on this consistency is largely anecdotal. Therefore there is a need first to measure this consistency among different laboratories and then identify and implement remedies. A pilot experiment to test logistics and provide initial data on consistency was therefore conceived. Methods. DNA aliquots at nominal concentrations between 10 and 300 ng/l were dispensed into the wells of 96-well plates by one participant - the coordinating centre. Participants estimated the concentration in each well and returned estimates to the coordinating centre. Results. Considerable overall variability was observed among estimates. There were statistically significant differences between participants' measurements and between fluorescence emission and absorption spectroscopy. Conclusion. Anecdotal evidence of variability in DNA concentration estimation has been substantiated. Reduction in variability between participants will require the identification of major sources of variation, specification of effective remedies and their implementation. © 2009 Yuille et al.

AB - Background. There is little confidence in the consistency of estimation of DNA concentrations when samples move between laboratories. Evidence on this consistency is largely anecdotal. Therefore there is a need first to measure this consistency among different laboratories and then identify and implement remedies. A pilot experiment to test logistics and provide initial data on consistency was therefore conceived. Methods. DNA aliquots at nominal concentrations between 10 and 300 ng/l were dispensed into the wells of 96-well plates by one participant - the coordinating centre. Participants estimated the concentration in each well and returned estimates to the coordinating centre. Results. Considerable overall variability was observed among estimates. There were statistically significant differences between participants' measurements and between fluorescence emission and absorption spectroscopy. Conclusion. Anecdotal evidence of variability in DNA concentration estimation has been substantiated. Reduction in variability between participants will require the identification of major sources of variation, specification of effective remedies and their implementation. © 2009 Yuille et al.

U2 - 10.1186/1756-0500-2-208

DO - 10.1186/1756-0500-2-208

M3 - Article

C2 - 19825187

SN - 1756-0500

VL - 2

JO - BMC Research Notes

JF - BMC Research Notes

M1 - 208

ER -

Observational study on variability between biobanks in the estimation of DNA concentration

Abstract

Access to Document

Fingerprint

Cite this