‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes

Faye Craven, Joana Silva, MD Segarra-Maset, Kun Huang, Peter Both, Julie Gough, Sabine Flitsch, Simon Webb

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    Abstract

    β(1,4)-Galactosyltransferase (β4Gal-T1) and T. cruzi trans-sialidase (TcTS) have been used in a ‘one-pot’ cascade to provide vesicles (liposomes) with a trisaccharide coating. These soluble enzymes catalysed the transfer of galactose then sialic acid onto a synthetic N-acetylglucolipid embedded in the bilayers. Clustering of this substrate into microdomains increased the rate of sialylated lipid production, showing that an increase in β4Gal-T1 activity is carried through the enzymatic cascade. These coatings modulated cell recognition. Hepatocellular carcinoma cells took up vesicles modified by β4Gal-T1 alone more extensively than sialylated vesicles produced by ‘one-pot’ sequential enzymatic modification.
    Original languageEnglish
    Pages (from-to)1347-1350
    Number of pages4
    JournalChemical Communications
    Volume54
    Issue number11
    Early online date4 Jan 2018
    DOIs
    Publication statusPublished - 2018

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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