Optical fibre biosensors for oxygen and glucose monitoring

J. S. Young, P. J. Scully, F. Kvasnik, K. Rose, G. Kuncova, O. Podrazky, V. Matejec, J. Mrazek

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER®) is described. The Ruthenium/ ORMOCER® layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.
    Original languageEnglish
    Title of host publicationProceedings of SPIE - The International Society for Optical Engineering|Proc SPIE Int Soc Opt Eng
    EditorsM. Voet, R. Willsch, W. Ecke, J. Jones, B. Culshaw
    PublisherSPIE
    Pages431-434
    Number of pages3
    Volume5855
    DOIs
    Publication statusPublished - 2005
    Event17th International Conference on Optical Fibre Sensors, OFS-17 - Bruges
    Duration: 1 Jul 2005 → …

    Conference

    Conference17th International Conference on Optical Fibre Sensors, OFS-17
    CityBruges
    Period1/07/05 → …

    Keywords

    • Fluorescence
    • Glucose
    • Lifetime
    • Optical fibre biosensor
    • ORMOCER®
    • Oxygen
    • POF
    • Ruthenium

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