Optical fibre biosensors for oxygen and glucose monitoring

J. S. Young; P. J. Scully; F. Kvasnik; K. Rose; G. Kuncova; O. Podrazky; V. Matejec; J. Mrazek

doi:10.1117/12.623688

Optical fibre biosensors for oxygen and glucose monitoring

J. S. Young, P. J. Scully, F. Kvasnik, K. Rose, G. Kuncova, O. Podrazky, V. Matejec, J. Mrazek

Research output: Chapter in Book/Conference proceeding › Conference contribution › peer-review

Abstract

An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER®) is described. The Ruthenium/ ORMOCER® layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.

Original language	English
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering\|Proc SPIE Int Soc Opt Eng
Editors	M. Voet, R. Willsch, W. Ecke, J. Jones, B. Culshaw
Publisher	SPIE
Pages	431-434
Number of pages	3
Volume	5855
DOIs	https://doi.org/10.1117/12.623688
Publication status	Published - 2005
Event	17th International Conference on Optical Fibre Sensors, OFS-17 - Bruges Duration: 1 Jul 2005 → …

Conference

Conference	17th International Conference on Optical Fibre Sensors, OFS-17
City	Bruges
Period	1/07/05 → …

Keywords

Fluorescence
Glucose
Lifetime
Optical fibre biosensor
ORMOCER®
Oxygen
POF
Ruthenium

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10.1117/12.623688

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Young, J. S., Scully, P. J., Kvasnik, F., Rose, K., Kuncova, G., Podrazky, O., Matejec, V., & Mrazek, J. (2005). Optical fibre biosensors for oxygen and glucose monitoring. In M. Voet, R. Willsch, W. Ecke, J. Jones, & B. Culshaw (Eds.), Proceedings of SPIE - The International Society for Optical Engineering|Proc SPIE Int Soc Opt Eng (Vol. 5855, pp. 431-434). SPIE. https://doi.org/10.1117/12.623688

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title = "Optical fibre biosensors for oxygen and glucose monitoring",

abstract = "An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER{\textregistered}) is described. The Ruthenium/ ORMOCER{\textregistered} layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.",

keywords = "Fluorescence, Glucose, Lifetime, Optical fibre biosensor, ORMOCER{\textregistered}, Oxygen, POF, Ruthenium",

author = "Young, {J. S.} and Scully, {P. J.} and F. Kvasnik and K. Rose and G. Kuncova and O. Podrazky and V. Matejec and J. Mrazek",

year = "2005",

doi = "10.1117/12.623688",

language = "English",

volume = "5855",

pages = "431--434",

editor = "M. Voet and R. Willsch and W. Ecke and J. Jones and B. Culshaw",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering|Proc SPIE Int Soc Opt Eng",

publisher = "SPIE",

address = "United States",

note = "17th International Conference on Optical Fibre Sensors, OFS-17 ; Conference date: 01-07-2005",

}

Young, JS, Scully, PJ, Kvasnik, F, Rose, K, Kuncova, G, Podrazky, O, Matejec, V & Mrazek, J 2005, Optical fibre biosensors for oxygen and glucose monitoring. in M Voet, R Willsch, W Ecke, J Jones & B Culshaw (eds), Proceedings of SPIE - The International Society for Optical Engineering|Proc SPIE Int Soc Opt Eng. vol. 5855, SPIE, pp. 431-434, 17th International Conference on Optical Fibre Sensors, OFS-17, Bruges, 1/07/05. https://doi.org/10.1117/12.623688

Optical fibre biosensors for oxygen and glucose monitoring. / Young, J. S.; Scully, P. J.; Kvasnik, F. et al.
Proceedings of SPIE - The International Society for Optical Engineering|Proc SPIE Int Soc Opt Eng. ed. / M. Voet; R. Willsch; W. Ecke; J. Jones; B. Culshaw. Vol. 5855 SPIE, 2005. p. 431-434.

Research output: Chapter in Book/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Optical fibre biosensors for oxygen and glucose monitoring

AU - Young, J. S.

AU - Scully, P. J.

AU - Kvasnik, F.

AU - Rose, K.

AU - Kuncova, G.

AU - Podrazky, O.

AU - Matejec, V.

AU - Mrazek, J.

PY - 2005

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N2 - An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER®) is described. The Ruthenium/ ORMOCER® layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.

AB - An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER®) is described. The Ruthenium/ ORMOCER® layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.

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KW - Glucose

KW - Lifetime

KW - Optical fibre biosensor

KW - ORMOCER®

KW - Oxygen

KW - POF

KW - Ruthenium

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DO - 10.1117/12.623688

M3 - Conference contribution

VL - 5855

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EP - 434

BT - Proceedings of SPIE - The International Society for Optical Engineering|Proc SPIE Int Soc Opt Eng

A2 - Voet, M.

A2 - Willsch, R.

A2 - Ecke, W.

A2 - Jones, J.

A2 - Culshaw, B.

PB - SPIE

T2 - 17th International Conference on Optical Fibre Sensors, OFS-17

Y2 - 1 July 2005

ER -

Optical fibre biosensors for oxygen and glucose monitoring

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Keywords

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