Abstract
An integrated, sensitive, and rapid system was developed for the detection of bacteria. The system combined an optical metal-clad leaky waveguide (MCLW) sensor with ultrasound standing waves (USW). The performance of a MCLW sensor for the detection of bacteria has been increased (>100 fold) by using USWs to drive bacteria onto the sensor surface. By forming the USW nodes at or within the surface of the MCLW, the diffusion-limited capture rate has been replaced by fast movement. Immobilized anti-BG antibody on the MCLW sensor surface was used to capture Bacillus subtilis var. niger (BG) bacterial spores driven to the surface. This combination of sensor and attractor force combination has been tested by detecting the evanescent scattering from bacterial spores at the sensor surface. Application of ultrasound for 3 min gave a detection limit for BG bacterial spores of 1 × 103 spores/mL. © 2005 American Chemical Society.
Original language | English |
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Pages (from-to) | 6163-6168 |
Number of pages | 5 |
Journal | Analytical Chemistry |
Volume | 77 |
Issue number | 19 |
DOIs | |
Publication status | Published - 1 Oct 2005 |
Keywords
- Optical sensors (MCLW (metal clad leaky waveguide)
- optical metal-clad leaky waveguide sensor with ultrasound standing waves for detection of bacteria with ultrasound attractor force and immobilized anti-bacteria antibody)
- Immobilization (antibody
- Optical waveguides (leaky
- Acoustic devices
- Attractive force
- Bacillus subtilis niger
- Diffusion
- Eubacteria
- Light scattering
- Separation (optical metal-clad leaky waveguide sensor with ultrasound standing waves for detection of bacteria with ultrasound attractor force and immobilized anti-bacteria antibody)
- Antibodies and Immunoglobulins Role: BUU (Biological use, unclassified), DEV (Device component use), BIOL (Biological study), USES (Uses) (optical metal-clad leaky waveguide sensor with ultrasound standing waves for detection of bacteria with ultrasound
- Biosensors (optical, MCLW (metal clad leaky waveguide)
- Sound and Ultrasound (standing waves
- Standing wave (ultrasound
- optical leaky waveguide sensor bacteria ultrasound attractor force