Optimization of cell seeding efficiencies on a three-dimensional gelatin scaffold for bone tissue engineering

G Jones, S H Cartmell

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Bone tissue engineering techniques hold great potential for the treatment of clin. defects. However, there is much optimization needed before bone tissue engineering can be used therapeutically. This study evaluated various cell seeding methods onto a porous three-dimensional (3D) scaffold for bone tissue engineering optimization. MG63 human osteoblast-like cells were seeded onto a resorbable, porous gelatin sponge in different suspension vols. (50 μl and 5 mL), and culture conditions, (static, shaken, rolled, or rotatory bioreactor). The DNA of the cells in the scaffold, the media and the containers were quantitated sep. to det. the cell no. and location after 3 days of culture. The samples were stained with calcein and viewed using confocal microscopy to det. cell viability and location. Placing a small cell suspension (50 μl) directly onto the scaffold produced a significantly higher proportion of cells adhered to the scaffold than a larger cell suspension (5 mL). In all conditions except the rotatory bioreactor, the percentage of cells remaining on the scaffold after 3 days in a small seeding vol. (63 ± 22%) was significantly higher than the larger seeding vol. (36 ± 25%). In the case of the rotatory bioreactor, the opposite appeared to be true (39 ± 9% small vol. and 72 ± 14% larger vol.). It was important to keep the seeding dynamics of the cultivated tissue engineered construct consistent throughout the expts. to ensure reproducibility. For this scaffold type, cells applied in a small vol. and cultured on a plate shaker at 120 rpm (giving 81 ± 14% of cells adhered to the scaffold) for 3 days is recommended. [on SciFinder(R)]
    Original languageEnglish
    Pages (from-to)172-180
    Number of pages9
    JournalJournal of Applied Biomaterials & Biomechanics
    Volume4
    Issue number3
    Publication statusPublished - 2006

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