Optimized methods for imaging membrane nanotubes between T cells and trafficking of HIV-1

Stefanie Sowinski; Juha Matti Alakoskela; Clare Jolly; Daniel M. Davis

doi:10.1016/j.ymeth.2010.04.002

Optimized methods for imaging membrane nanotubes between T cells and trafficking of HIV-1

Stefanie Sowinski, Juha Matti Alakoskela, Clare Jolly, Daniel M. Davis

Research output: Contribution to journal › Article › peer-review

Abstract

A wide variety of cell types, including immune cells, have been observed to frequently interact via transient, long-distance membrane connections [1-17]. However, considerable heterogeneity in their structure, mode of formation and functional properties has emerged, suggesting the existence of distinct subclasses [18-21]. Open-ended tunneling nanotubes allow for the trafficking of cytoplasmic material, e.g. endocytic vesicles, or the transmission of calcium signals [1,8]. Closed-ended membrane nanotubes do not seamlessly connect the cytoplasm between two interacting cells and a junction exists within the nanotube or where the nanotube meets a cell body [4,5,7]. Recent live cell imaging suggested that membrane nanotubes between T cells could present a novel route for HIV-1 transmission [7,22]. Here, we describe detailed protocols for observing membrane nanotubes and HIV-1 trafficking by live cell fluorescence microscopy. © 2010 Elsevier Inc.

Original language	English
Pages (from-to)	27-33
Number of pages	6
Journal	Methods
Volume	53
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2010.04.002
Publication status	Published - Jan 2011

Keywords

Cytoplasmic bridges
Filopodia
Gag-GFP
Laser scanning confocal microscopy
Live cell imaging
Membrane nanotubes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ymeth.2010.04.002

Cite this

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abstract = "A wide variety of cell types, including immune cells, have been observed to frequently interact via transient, long-distance membrane connections [1-17]. However, considerable heterogeneity in their structure, mode of formation and functional properties has emerged, suggesting the existence of distinct subclasses [18-21]. Open-ended tunneling nanotubes allow for the trafficking of cytoplasmic material, e.g. endocytic vesicles, or the transmission of calcium signals [1,8]. Closed-ended membrane nanotubes do not seamlessly connect the cytoplasm between two interacting cells and a junction exists within the nanotube or where the nanotube meets a cell body [4,5,7]. Recent live cell imaging suggested that membrane nanotubes between T cells could present a novel route for HIV-1 transmission [7,22]. Here, we describe detailed protocols for observing membrane nanotubes and HIV-1 trafficking by live cell fluorescence microscopy. {\textcopyright} 2010 Elsevier Inc.",

keywords = "Cytoplasmic bridges, Filopodia, Gag-GFP, Laser scanning confocal microscopy, Live cell imaging, Membrane nanotubes",

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AU - Jolly, Clare

AU - Davis, Daniel M.

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KW - Cytoplasmic bridges

KW - Filopodia

KW - Gag-GFP

KW - Laser scanning confocal microscopy

KW - Live cell imaging

KW - Membrane nanotubes

U2 - 10.1016/j.ymeth.2010.04.002

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JF - Methods

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Optimized methods for imaging membrane nanotubes between T cells and trafficking of HIV-1

Abstract

Keywords

UN SDGs

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