Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Ellen M Quardokus; Diane C Saunders; Elizabeth McDonough; John W Hickey; Christopher Werlein; Christine Surrette; Presha Rajbhandari; Anna Martinez Casals; Hua Tian; Lisa Lowery; Elizabeth K Neumann; Frida Björklund; Taruna V Neelakantan; Josh Croteau; Anne E Wiblin; Jeremy Fisher; April J Livengood; Karen G Dowell; Jonathan C Silverstein; Jeffrey M Spraggins; Gloria S Pryhuber; Gail Deutsch; Fiona Ginty; Garry P Nolan; Simon Melov; Danny Jonigk; Michael A Caldwell; Ioannis S Vlachos; Werner Muller; Nils Gehlenborg; Brent R Stockwell; Emma Lundberg; Michael P Snyder; Ronald N Germain; Jeannie M Camarillo; Neil L Kelleher; Katy Börner; Andrea J Radtke

doi:10.1038/s41592-023-01846-7

Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Ellen M Quardokus, Diane C Saunders, Elizabeth McDonough, John W Hickey, Christopher Werlein, Christine Surrette, Presha Rajbhandari, Anna Martinez Casals, Hua Tian, Lisa Lowery, Elizabeth K Neumann, Frida Björklund, Taruna V Neelakantan, Josh Croteau, Anne E Wiblin, Jeremy Fisher, April J Livengood, Karen G Dowell, Jonathan C Silverstein, Jeffrey M SpragginsGloria S Pryhuber, Gail Deutsch, Fiona Ginty, Garry P Nolan, Simon Melov, Danny Jonigk, Michael A Caldwell, Ioannis S Vlachos, Werner Muller, Nils Gehlenborg, Brent R Stockwell, Emma Lundberg, Michael P Snyder, Ronald N Germain, Jeannie M Camarillo, Neil L Kelleher, Katy Börner, Andrea J Radtke

Division of Immunology, Immunity to Infection and Respiratory Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

Original language	English
Pages (from-to)	1174-1178
Number of pages	5
Journal	Nature Methods
Volume	20
Issue number	8
Early online date	19 Jul 2023
DOIs	https://doi.org/10.1038/s41592-023-01846-7
Publication status	Published - 1 Aug 2023

Keywords

Spatial Biology
OMAP

Access to Document

10.1038/s41592-023-01846-7Licence: CC BY

https://www.ncbi.nlm.nih.gov/pubmed/37468619

Cite this

Quardokus, E. M., Saunders, D. C., McDonough, E., Hickey, J. W., Werlein, C., Surrette, C., Rajbhandari, P., Casals, A. M., Tian, H., Lowery, L., Neumann, E. K., Björklund, F., Neelakantan, T. V., Croteau, J., Wiblin, A. E., Fisher, J., Livengood, A. J., Dowell, K. G., Silverstein, J. C., ... Radtke, A. J. (2023). Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging. Nature Methods, 20(8), 1174-1178. https://doi.org/10.1038/s41592-023-01846-7

@article{47a89d54a6e94508987804508f1ab73b,

title = "Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging",

abstract = "Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.",

keywords = "Spatial Biology, OMAP",

author = "Quardokus, {Ellen M} and Saunders, {Diane C} and Elizabeth McDonough and Hickey, {John W} and Christopher Werlein and Christine Surrette and Presha Rajbhandari and Casals, {Anna Martinez} and Hua Tian and Lisa Lowery and Neumann, {Elizabeth K} and Frida Bj{\"o}rklund and Neelakantan, {Taruna V} and Josh Croteau and Wiblin, {Anne E} and Jeremy Fisher and Livengood, {April J} and Dowell, {Karen G} and Silverstein, {Jonathan C} and Spraggins, {Jeffrey M} and Pryhuber, {Gloria S} and Gail Deutsch and Fiona Ginty and Nolan, {Garry P} and Simon Melov and Danny Jonigk and Caldwell, {Michael A} and Vlachos, {Ioannis S} and Werner Muller and Nils Gehlenborg and Stockwell, {Brent R} and Emma Lundberg and Snyder, {Michael P} and Germain, {Ronald N} and Camarillo, {Jeannie M} and Kelleher, {Neil L} and Katy B{\"o}rner and Radtke, {Andrea J}",

year = "2023",

month = aug,

day = "1",

doi = "10.1038/s41592-023-01846-7",

language = "English",

volume = "20",

pages = "1174--1178",

journal = "Nature Methods",

issn = "1548-7105",

publisher = "Springer Nature",

number = "8",

}

Quardokus, EM, Saunders, DC, McDonough, E, Hickey, JW, Werlein, C, Surrette, C, Rajbhandari, P, Casals, AM, Tian, H, Lowery, L, Neumann, EK, Björklund, F, Neelakantan, TV, Croteau, J, Wiblin, AE, Fisher, J, Livengood, AJ, Dowell, KG, Silverstein, JC, Spraggins, JM, Pryhuber, GS, Deutsch, G, Ginty, F, Nolan, GP, Melov, S, Jonigk, D, Caldwell, MA, Vlachos, IS, Muller, W, Gehlenborg, N, Stockwell, BR, Lundberg, E, Snyder, MP, Germain, RN, Camarillo, JM, Kelleher, NL, Börner, K & Radtke, AJ 2023, 'Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging', Nature Methods, vol. 20, no. 8, pp. 1174-1178. https://doi.org/10.1038/s41592-023-01846-7

TY - JOUR

T1 - Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

AU - Quardokus, Ellen M

AU - Saunders, Diane C

AU - McDonough, Elizabeth

AU - Hickey, John W

AU - Werlein, Christopher

AU - Surrette, Christine

AU - Rajbhandari, Presha

AU - Casals, Anna Martinez

AU - Tian, Hua

AU - Lowery, Lisa

AU - Neumann, Elizabeth K

AU - Björklund, Frida

AU - Neelakantan, Taruna V

AU - Croteau, Josh

AU - Wiblin, Anne E

AU - Fisher, Jeremy

AU - Livengood, April J

AU - Dowell, Karen G

AU - Silverstein, Jonathan C

AU - Spraggins, Jeffrey M

AU - Pryhuber, Gloria S

AU - Deutsch, Gail

AU - Ginty, Fiona

AU - Nolan, Garry P

AU - Melov, Simon

AU - Jonigk, Danny

AU - Caldwell, Michael A

AU - Vlachos, Ioannis S

AU - Muller, Werner

AU - Gehlenborg, Nils

AU - Stockwell, Brent R

AU - Lundberg, Emma

AU - Snyder, Michael P

AU - Germain, Ronald N

AU - Camarillo, Jeannie M

AU - Kelleher, Neil L

AU - Börner, Katy

AU - Radtke, Andrea J

PY - 2023/8/1

Y1 - 2023/8/1

N2 - Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

AB - Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

KW - Spatial Biology

KW - OMAP

UR - http://www.scopus.com/inward/record.url?scp=85165169913&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/ba0d58a1-160c-3dc5-ba58-ff83a5a4b743/

U2 - 10.1038/s41592-023-01846-7

DO - 10.1038/s41592-023-01846-7

M3 - Article

C2 - 37468619

SN - 1548-7105

VL - 20

SP - 1174

EP - 1178

JO - Nature Methods

JF - Nature Methods

IS - 8

ER -

Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this