Orthogonal Regulatory Circuits for Escherichia coli Based on the gamma-Butyrolactone System of Streptomyces coelicolor

Marc Biarnes-Carrera, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, Eriko Takano

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    Abstract

    Chemically inducible transcription factors are widely used to control gene expression of synthetic devices. The bacterial quorum sensing system is a popular tool to achieve such control. However, different quorum sensing systems have been found to cross-talk, both between themselves and with the hosts of these devices, and they are leaky by nature. Here we evaluate the potential use of the γ-butyolactone system from Streptomyces coelicolor A3(2) M145 as a complementary regulatory circuit. First, two additional genes responsible for the biosynthesis of γ-butyrolactones were identified in S. coelicolor M145 and then expressed in E. coli BL21 under various experimental conditions. Second, the γ-butyrolactone receptor ScbR was optimised for expression in E. coli BL21. Finally, signal and promoter crosstalk between the γ-butyrolactone system from S. coelicolor and quorum sensing systems from Vibrio fischeri and Pseudomonas aeruginosa was evaluated. The results show that the γ-butyrolactone system does not cros...
    Original languageEnglish
    Pages (from-to)1043-1055
    Number of pages13
    JournalACS Synthetic Biology
    Volume7
    Issue number4
    Early online date6 Mar 2018
    DOIs
    Publication statusPublished - 20 Apr 2018

    Keywords

    • Streptomyces
    • orthogonal regulatory circuit
    • quorum sensing
    • synthetic biology
    • γ-butyrolactone

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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