Osteogenic differentiation of adipose-derived mesenchymal stem cells into Polycaprolactone (PCL) scaffold

G F Caetano; Paulo Jorge Da Silva bartolo; Marco Domingos; C C Oliveira; M N Leite; M A Frade

doi:10.1016/j.proeng.2015.07.010

Osteogenic differentiation of adipose-derived mesenchymal stem cells into Polycaprolactone (PCL) scaffold

G F Caetano
, Paulo Jorge Da Silva bartolo
, Marco Domingos
, C C Oliveira
, M N Leite
, M A Frade

Research output: Contribution to journal › Article › peer-review

Abstract

The objective of this study was to evaluate the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) into polycaprolactone scaffold (PCL). After ADSC characterization, cells were seeded onto the scaffolds and incubated with basal or osteogenic differentiating medium to MTT assay and osteogenesis evaluation. MTT cell viability assay showedthatcell-seeded scaffolds cultured in both mediums presented formazan cristals formed as a result of the presence of viable cells. The osteogenic assay showedthatcell-seeded PCL scaffolds cultured in osteogenic medium presentedsignificantly greater amount of staining dye when compared to basal medium and scaffold without cells.The PCL scaffoldhave great potential to be usedassociated to ADSCs in Tissue Engineering to bone tissue regeneration

Original language	English
Pages (from-to)	59-66
Number of pages	7
Journal	Procedia Engineering
Volume	110
DOIs	https://doi.org/10.1016/j.proeng.2015.07.010
Publication status	Published - 2015

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title = "Osteogenic differentiation of adipose-derived mesenchymal stem cells into Polycaprolactone (PCL) scaffold",

abstract = "The objective of this study was to evaluate the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) into polycaprolactone scaffold (PCL). After ADSC characterization, cells were seeded onto the scaffolds and incubated with basal or osteogenic differentiating medium to MTT assay and osteogenesis evaluation. MTT cell viability assay showedthatcell-seeded scaffolds cultured in both mediums presented formazan cristals formed as a result of the presence of viable cells. The osteogenic assay showedthatcell-seeded PCL scaffolds cultured in osteogenic medium presentedsignificantly greater amount of staining dye when compared to basal medium and scaffold without cells.The PCL scaffoldhave great potential to be usedassociated to ADSCs in Tissue Engineering to bone tissue regeneration",

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year = "2015",

doi = "10.1016/j.proeng.2015.07.010",

language = "English",

volume = "110",

pages = "59--66",

journal = "Procedia Engineering",

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T1 - Osteogenic differentiation of adipose-derived mesenchymal stem cells into Polycaprolactone (PCL) scaffold

AU - Caetano, G F

AU - Da Silva bartolo, Paulo Jorge

AU - Domingos, Marco

AU - Oliveira, C C

AU - Leite, M N

AU - Frade, M A

PY - 2015

Y1 - 2015

N2 - The objective of this study was to evaluate the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) into polycaprolactone scaffold (PCL). After ADSC characterization, cells were seeded onto the scaffolds and incubated with basal or osteogenic differentiating medium to MTT assay and osteogenesis evaluation. MTT cell viability assay showedthatcell-seeded scaffolds cultured in both mediums presented formazan cristals formed as a result of the presence of viable cells. The osteogenic assay showedthatcell-seeded PCL scaffolds cultured in osteogenic medium presentedsignificantly greater amount of staining dye when compared to basal medium and scaffold without cells.The PCL scaffoldhave great potential to be usedassociated to ADSCs in Tissue Engineering to bone tissue regeneration

AB - The objective of this study was to evaluate the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) into polycaprolactone scaffold (PCL). After ADSC characterization, cells were seeded onto the scaffolds and incubated with basal or osteogenic differentiating medium to MTT assay and osteogenesis evaluation. MTT cell viability assay showedthatcell-seeded scaffolds cultured in both mediums presented formazan cristals formed as a result of the presence of viable cells. The osteogenic assay showedthatcell-seeded PCL scaffolds cultured in osteogenic medium presentedsignificantly greater amount of staining dye when compared to basal medium and scaffold without cells.The PCL scaffoldhave great potential to be usedassociated to ADSCs in Tissue Engineering to bone tissue regeneration

U2 - 10.1016/j.proeng.2015.07.010

DO - 10.1016/j.proeng.2015.07.010

M3 - Article

SN - 1877-7058

VL - 110

SP - 59

EP - 66

JO - Procedia Engineering

JF - Procedia Engineering

ER -

Osteogenic differentiation of adipose-derived mesenchymal stem cells into Polycaprolactone (PCL) scaffold

Abstract

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