OTX2 homeodomain protein binds a DNA element necessary for interphotoreceptor retinoid binding protein gene expression

Nicoletta Bobola; Paola Briata; Cristina Ilengo; Nadia Rosatto; Cheryl Craft; Giorgio Corte; Roberto Ravazzolo

doi:10.1016/S0925-4773(98)00162-2

OTX2 homeodomain protein binds a DNA element necessary for interphotoreceptor retinoid binding protein gene expression

Nicoletta Bobola, Paola Briata, Cristina Ilengo, Nadia Rosatto, Cheryl Craft, Giorgio Corte, Roberto Ravazzolo

Research output: Contribution to journal › Article › peer-review

Abstract

Transcription of the human interphotoreceptor retinoid binding protein (IRBP) gene is strictly tissue specific, being restricted to retinal photoreceptors and pinealocytes. We have previously demonstrated that a sequence named A element, in the IRBP promoter is essential for IRBP gene transcription in vivo. Here we demonstrate that the human homeodomain protein OTX2 is present in nuclear extracts of IRBP expressing cells and specifically interacts with the IRBP A promoter element in vitro. OTX2, as well as CRX, a homeodomain protein very similar to OTX2, activates the human IRBP promoter in co-transfection experiments.

Original language	English
Pages (from-to)	165-169
Number of pages	4
Journal	Mechanisms of Development
Volume	82
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(98)00162-2
Publication status	Published - 1 Apr 1999

Keywords

Bicoid
CRX
Developmen t
Dro sophila
Eye
Gel retardation
Gene
HeLa
Homeodomain
Human
Immunohistochemistry
Interphotoreceptor retinoid binding protein
IRBP
OTX2
Photoreceptors
Promoter
Retina
Retinoblastoma
Supershift
Transcriptional regulation
Transfection
Vertebrate
WERI- Rb1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0925-4773(98)00162-2

Cite this

@article{4866eb6ea56a45edbeff4f477739b499,

title = "OTX2 homeodomain protein binds a DNA element necessary for interphotoreceptor retinoid binding protein gene expression",

abstract = "Transcription of the human interphotoreceptor retinoid binding protein (IRBP) gene is strictly tissue specific, being restricted to retinal photoreceptors and pinealocytes. We have previously demonstrated that a sequence named A element, in the IRBP promoter is essential for IRBP gene transcription in vivo. Here we demonstrate that the human homeodomain protein OTX2 is present in nuclear extracts of IRBP expressing cells and specifically interacts with the IRBP A promoter element in vitro. OTX2, as well as CRX, a homeodomain protein very similar to OTX2, activates the human IRBP promoter in co-transfection experiments.",

keywords = "Bicoid, CRX, Developmen t, Dro sophila, Eye, Gel retardation, Gene, HeLa, Homeodomain, Human, Immunohistochemistry, Interphotoreceptor retinoid binding protein, IRBP, OTX2, Photoreceptors, Promoter, Retina, Retinoblastoma, Supershift, Transcriptional regulation, Transfection, Vertebrate, WERI- Rb1",

author = "Nicoletta Bobola and Paola Briata and Cristina Ilengo and Nadia Rosatto and Cheryl Craft and Giorgio Corte and Roberto Ravazzolo",

year = "1999",

month = apr,

day = "1",

doi = "10.1016/S0925-4773(98)00162-2",

language = "English",

volume = "82",

pages = "165--169",

journal = "Mechanisms of Development",

issn = "0925-4773",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - OTX2 homeodomain protein binds a DNA element necessary for interphotoreceptor retinoid binding protein gene expression

AU - Bobola, Nicoletta

AU - Briata, Paola

AU - Ilengo, Cristina

AU - Rosatto, Nadia

AU - Craft, Cheryl

AU - Corte, Giorgio

AU - Ravazzolo, Roberto

PY - 1999/4/1

Y1 - 1999/4/1

N2 - Transcription of the human interphotoreceptor retinoid binding protein (IRBP) gene is strictly tissue specific, being restricted to retinal photoreceptors and pinealocytes. We have previously demonstrated that a sequence named A element, in the IRBP promoter is essential for IRBP gene transcription in vivo. Here we demonstrate that the human homeodomain protein OTX2 is present in nuclear extracts of IRBP expressing cells and specifically interacts with the IRBP A promoter element in vitro. OTX2, as well as CRX, a homeodomain protein very similar to OTX2, activates the human IRBP promoter in co-transfection experiments.

AB - Transcription of the human interphotoreceptor retinoid binding protein (IRBP) gene is strictly tissue specific, being restricted to retinal photoreceptors and pinealocytes. We have previously demonstrated that a sequence named A element, in the IRBP promoter is essential for IRBP gene transcription in vivo. Here we demonstrate that the human homeodomain protein OTX2 is present in nuclear extracts of IRBP expressing cells and specifically interacts with the IRBP A promoter element in vitro. OTX2, as well as CRX, a homeodomain protein very similar to OTX2, activates the human IRBP promoter in co-transfection experiments.

KW - Bicoid

KW - CRX

KW - Developmen t

KW - Dro sophila

KW - Eye

KW - Gel retardation

KW - Gene

KW - HeLa

KW - Homeodomain

KW - Human

KW - Immunohistochemistry

KW - Interphotoreceptor retinoid binding protein

KW - IRBP

KW - OTX2

KW - Photoreceptors

KW - Promoter

KW - Retina

KW - Retinoblastoma

KW - Supershift

KW - Transcriptional regulation

KW - Transfection

KW - Vertebrate

KW - WERI- Rb1

U2 - 10.1016/S0925-4773(98)00162-2

DO - 10.1016/S0925-4773(98)00162-2

M3 - Article

SN - 0925-4773

VL - 82

SP - 165

EP - 169

JO - Mechanisms of Development

JF - Mechanisms of Development

IS - 1-2

ER -

OTX2 homeodomain protein binds a DNA element necessary for interphotoreceptor retinoid binding protein gene expression

Abstract

Keywords

UN SDGs

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