Ovariectomy enhances SR Ca 2+ release and increases Ca 2+ spark amplitudes in isolated ventricular myocytes

Elias Fares; Randi J. Parks; Jennifer K. MacDonald; Jeanne M S Egar; Susan E. Howlett

doi:10.1016/j.yjmcc.2011.09.002

Ovariectomy enhances SR Ca 2+ release and increases Ca 2+ spark amplitudes in isolated ventricular myocytes

Elias Fares, Randi J. Parks, Jennifer K. MacDonald, Jeanne M S Egar, Susan E. Howlett

Research output: Contribution to journal › Article › peer-review

Abstract

This study compared Ca 2+ homeostasis in ventricular myocytes from 8-month-old female C57BL/6J mice that had either a bilateral ovariectomy (OVX) or a sham surgery at 3weeks of age. Cells were loaded with fura-2 and field-stimulated or voltage-clamped with steps to membrane potentials between -40 and +80mV (37°C). Peak Ca 2+ transients increased by two-fold in OVX myocytes when compared to sham, and Ca 2+ transient rates of rise and decay were faster in OVX cells. In contrast, Ca 2+ current densities were similar in sham and OVX cells. Sarcoplasmic reticulum (SR) Ca 2+ content, assessed by caffeine, also was higher in OVX compared to sham cells (111.7±11.9 vs. 61.2±10.4nM; p

Original language	English
Pages (from-to)	32-42
Number of pages	10
Journal	Journal of molecular and cellular cardiology
Volume	52
Issue number	1
DOIs	https://doi.org/10.1016/j.yjmcc.2011.09.002
Publication status	Published - Jan 2012

Keywords

Calcium transients
Estrogen
Excitation-contraction coupling
Gender
Sex differences

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10.1016/j.yjmcc.2011.09.002

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title = "Ovariectomy enhances SR Ca 2+ release and increases Ca 2+ spark amplitudes in isolated ventricular myocytes",

abstract = "This study compared Ca 2+ homeostasis in ventricular myocytes from 8-month-old female C57BL/6J mice that had either a bilateral ovariectomy (OVX) or a sham surgery at 3weeks of age. Cells were loaded with fura-2 and field-stimulated or voltage-clamped with steps to membrane potentials between -40 and +80mV (37°C). Peak Ca 2+ transients increased by two-fold in OVX myocytes when compared to sham, and Ca 2+ transient rates of rise and decay were faster in OVX cells. In contrast, Ca 2+ current densities were similar in sham and OVX cells. Sarcoplasmic reticulum (SR) Ca 2+ content, assessed by caffeine, also was higher in OVX compared to sham cells (111.7±11.9 vs. 61.2±10.4nM; p",

keywords = "Calcium transients, Estrogen, Excitation-contraction coupling, Gender, Sex differences",

author = "Elias Fares and Parks, {Randi J.} and MacDonald, {Jennifer K.} and Egar, {Jeanne M S} and Howlett, {Susan E.}",

note = ", Canadian Institutes of Health Research, Canada",

year = "2012",

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doi = "10.1016/j.yjmcc.2011.09.002",

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T1 - Ovariectomy enhances SR Ca 2+ release and increases Ca 2+ spark amplitudes in isolated ventricular myocytes

AU - Fares, Elias

AU - Parks, Randi J.

AU - MacDonald, Jennifer K.

AU - Egar, Jeanne M S

AU - Howlett, Susan E.

N1 - , Canadian Institutes of Health Research, Canada

PY - 2012/1

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KW - Calcium transients

KW - Estrogen

KW - Excitation-contraction coupling

KW - Gender

KW - Sex differences

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Ovariectomy enhances SR Ca 2+ release and increases Ca 2+ spark amplitudes in isolated ventricular myocytes

Abstract

Keywords

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