Ovariectomy enhances SR Ca 2+ release and increases Ca 2+ spark amplitudes in isolated ventricular myocytes

Elias Fares, Randi J. Parks, Jennifer K. MacDonald, Jeanne M S Egar, Susan E. Howlett

    Research output: Contribution to journalArticlepeer-review

    Abstract

    This study compared Ca 2+ homeostasis in ventricular myocytes from 8-month-old female C57BL/6J mice that had either a bilateral ovariectomy (OVX) or a sham surgery at 3weeks of age. Cells were loaded with fura-2 and field-stimulated or voltage-clamped with steps to membrane potentials between -40 and +80mV (37°C). Peak Ca 2+ transients increased by two-fold in OVX myocytes when compared to sham, and Ca 2+ transient rates of rise and decay were faster in OVX cells. In contrast, Ca 2+ current densities were similar in sham and OVX cells. Sarcoplasmic reticulum (SR) Ca 2+ content, assessed by caffeine, also was higher in OVX compared to sham cells (111.7±11.9 vs. 61.2±10.4nM; p
    Original languageEnglish
    Pages (from-to)32-42
    Number of pages10
    JournalJournal of molecular and cellular cardiology
    Volume52
    Issue number1
    DOIs
    Publication statusPublished - Jan 2012

    Keywords

    • Calcium transients
    • Estrogen
    • Excitation-contraction coupling
    • Gender
    • Sex differences

    Fingerprint

    Dive into the research topics of 'Ovariectomy enhances SR Ca 2+ release and increases Ca 2+ spark amplitudes in isolated ventricular myocytes'. Together they form a unique fingerprint.

    Cite this