Abstract
Pancreatitis is an inflammatory disease of pancreatic acinar cells whereby intracellular calcium concentration ([Ca2+]i) signaling and enzyme secretion are impaired. Increased oxidative stress has been suggested to mediate the associated cell injury. The present study tested the effects of the oxidant, hydrogen peroxide, on [Ca2+]i signaling in rat pancreatic acinar cells by simultaneously imaging fura-2, to measure [Ca2+]i, and dichlorofluorescein, to measure oxidative stress. Millimolar concentrations of hydrogen peroxide increased cellular oxidative stress and irreversibly increased [Ca2+]i, which was sensitive to antioxidants and removal of external Ca2+, and ultimately led to cell lysis. Responses were also abolished by pretreatment with (sarco)endoplasmic reticulum Ca2+-ATPase inhibitors, unless cells were prestimulated with cholecystokinin to promote mitochondrial Ca2+ uptake. This suggests that hydrogen peroxide promotes Ca2+ release from the endoplasmic reticulum and the mitochondria and that it promotes Ca 2+ influx. Lower concentrations of hydrogen peroxide (10-100 μM) increased [Ca2+]i and altered cholecystokinin-evoked [Ca2+]i oscillations with marked heterogeneity, the severity of which was directly related to oxidative stress, suggesting differences in cellular antioxidant capacity. These changes in [Ca 2+]i also upregulated the activity of the plasma membrane Ca2+-ATPase in a Ca2+-dependent manner, whereas higher concentrations (0.1-1 mM) inactivated the plasma membrane Ca2+- ATPase. This may be important in facilitating "Ca2+ overload," resulting in cell injury associated with pancreatitis. Copyright © 2007 the American Physiological Society.
Original language | English |
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Pages (from-to) | C938-C950 |
Journal | American Journal of Physiology: Cell Physiology |
Volume | 293 |
Issue number | 3 |
DOIs | |
Publication status | Published - Sept 2007 |
Keywords
- Calcium pump
- Oxidant stress
- Pancreatitis