P450 BM3: The very model of a modern flavocytochrome

Andrew W. Munro; David G. Leys; Kirsty J. McLean; Ker R. Marshall; Tobias W B Ost; Simon Daff; Caroline S. Miles; Stephen K. Chapman; Dominikus A. Lysek; Christopher C. Moser; Christopher C. Page; P. Leslie Dutton

doi:10.1016/S0968-0004(02)02086-8

P450 BM3: The very model of a modern flavocytochrome

Andrew W. Munro, David G. Leys, Kirsty J. McLean, Ker R. Marshall, Tobias W B Ost, Simon Daff, Caroline S. Miles, Stephen K. Chapman, Dominikus A. Lysek, Christopher C. Moser, Christopher C. Page, P. Leslie Dutton

Research output: Contribution to journal › Article › peer-review

Abstract

Flavocytochrome P450 BM3 is a bacterial P450 system in which a fatty acid hydroxylase P450 is fused to a mammalian-like diflavin NADPH-P450 reductase in a single polypeptide. The enzyme is soluble (unlike mammalian P450 redox systems) and its fusion arrangement affords it the highest catalytic activity of any P450 mono-oxygenase. This article discusses the fundamental properties of P450 BM3 and how progress with this model P450 has affected our comprehension of P450 systems in general.

Original language	English
Pages (from-to)	250-257
Number of pages	7
Journal	Trends in Biochemical Sciences
Volume	27
Issue number	5
DOIs	https://doi.org/10.1016/S0968-0004(02)02086-8
Publication status	Published - 1 May 2002

Access to Document

10.1016/S0968-0004(02)02086-8

Cite this

@article{9ab0b7e3c51546b982c099efcbd9c68b,

title = "P450 BM3: The very model of a modern flavocytochrome",

abstract = "Flavocytochrome P450 BM3 is a bacterial P450 system in which a fatty acid hydroxylase P450 is fused to a mammalian-like diflavin NADPH-P450 reductase in a single polypeptide. The enzyme is soluble (unlike mammalian P450 redox systems) and its fusion arrangement affords it the highest catalytic activity of any P450 mono-oxygenase. This article discusses the fundamental properties of P450 BM3 and how progress with this model P450 has affected our comprehension of P450 systems in general.",

author = "Munro, {Andrew W.} and Leys, {David G.} and McLean, {Kirsty J.} and Marshall, {Ker R.} and Ost, {Tobias W B} and Simon Daff and Miles, {Caroline S.} and Chapman, {Stephen K.} and Lysek, {Dominikus A.} and Moser, {Christopher C.} and Page, {Christopher C.} and Dutton, {P. Leslie}",

year = "2002",

month = may,

day = "1",

doi = "10.1016/S0968-0004(02)02086-8",

language = "English",

volume = "27",

pages = "250--257",

journal = "Trends in Biochemical Sciences",

issn = "0968-0004",

publisher = "Elsevier BV",

number = "5",

}

TY - JOUR

T1 - P450 BM3: The very model of a modern flavocytochrome

AU - Munro, Andrew W.

AU - Leys, David G.

AU - McLean, Kirsty J.

AU - Marshall, Ker R.

AU - Ost, Tobias W B

AU - Daff, Simon

AU - Miles, Caroline S.

AU - Chapman, Stephen K.

AU - Lysek, Dominikus A.

AU - Moser, Christopher C.

AU - Page, Christopher C.

AU - Dutton, P. Leslie

PY - 2002/5/1

Y1 - 2002/5/1

N2 - Flavocytochrome P450 BM3 is a bacterial P450 system in which a fatty acid hydroxylase P450 is fused to a mammalian-like diflavin NADPH-P450 reductase in a single polypeptide. The enzyme is soluble (unlike mammalian P450 redox systems) and its fusion arrangement affords it the highest catalytic activity of any P450 mono-oxygenase. This article discusses the fundamental properties of P450 BM3 and how progress with this model P450 has affected our comprehension of P450 systems in general.

AB - Flavocytochrome P450 BM3 is a bacterial P450 system in which a fatty acid hydroxylase P450 is fused to a mammalian-like diflavin NADPH-P450 reductase in a single polypeptide. The enzyme is soluble (unlike mammalian P450 redox systems) and its fusion arrangement affords it the highest catalytic activity of any P450 mono-oxygenase. This article discusses the fundamental properties of P450 BM3 and how progress with this model P450 has affected our comprehension of P450 systems in general.

U2 - 10.1016/S0968-0004(02)02086-8

DO - 10.1016/S0968-0004(02)02086-8

M3 - Article

C2 - 12076537

SN - 0968-0004

VL - 27

SP - 250

EP - 257

JO - Trends in Biochemical Sciences

JF - Trends in Biochemical Sciences

IS - 5

ER -

P450 BM3: The very model of a modern flavocytochrome

Abstract

Access to Document

Fingerprint

Cite this