Palladium(II)-mediated assembly of biotinylated ion channels

Craig P. Wilson, Cécile Boglio, Long Ma, Scott L. Cockroft, Simon J. Webb

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Simple synthetic methodology has been used to create biotinylated pyridyl cholate lipids that can undergo multiple self-assembly events when inserted into phospholipid vesicles; PdII links cholates into transmembrane lipids, while avidin laterally clusters these complexes together and concomitantly assembles the vesicles into aggregates. The transmembrane assembly of cholates by PdII "opened" the ion channels, whereas avidin addition produced vesicle aggregates, giving a system that mimicked both transmembrane transport and cellular adhesion. Complexation of these Pd II-linked cholates by avidin gave a measurable decrease in ion flow, suggesting some channels became blocked or were prevented from adopting the optimum geometry for ion conduction. This reflects the importance of spatially appropriate preorganisation when generating active supramolecular assemblies. Half is not better than the hole: Functionalization of pyridyl-cholate lipids with biotin gave "sticky" palladium(II)-gated ion channels that also mediated vesicle-vesicle adhesion. Several levels of self-assembly were involved in the functional system: PdII-mediated transmembrane assembly of the lipids, avidin-induced intramembrane clustering of lipids (black arrow), and intermembrane adhesion (white arrow) into vesicle aggregates (top). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Original languageEnglish
    Pages (from-to)3465-3473
    Number of pages8
    JournalChemistry - A European Journal
    Volume17
    Issue number12
    DOIs
    Publication statusPublished - 14 Mar 2011

    Keywords

    • ion channels
    • membranes
    • molecular recognition
    • self assembly
    • vesicles

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