Paper Spray Ionization Ion Mobility Mass Spectrometry of Sebum Classifies Biomarker Classes for the Diagnosis of Parkinson's Disease

Depanjan Sarkar, Eleanor Sinclair, Sze Hway Lim, Caitlin Walton-Doyle, Kaneez Jafri, Joy Milne, Johannes P.C. Vissers, Keith Richardson, Drupad K. Trivedi, Monty Silverdale, Perdita Barran

Research output: Contribution to journalArticlepeer-review

Abstract

Parkinson's disease (PD) is the second most common neurodegenerative disorder, and identification of robust biomarkers to complement clinical diagnosis will accelerate treatment options. Here, we demonstrate the use of direct infusion of sebum from skin swabs using paper spray ionization coupled with ion mobility mass spectrometry (PS-IM-MS) to determine the regulation of molecular classes of lipids in sebum that are diagnostic of PD. A PS-IM-MS method for sebum samples that takes 3 min per swab was developed and optimized. The method was applied to skin swabs collected from 150 people and elucidates ∼4200 features from each subject, which were independently analyzed. The data included high molecular weight lipids (>600 Da) that differ significantly in the sebum of people with PD. Putative metabolite annotations of several lipid classes, predominantly triglycerides and larger acyl glycerides, were obtained using accurate mass, tandem mass spectrometry, and collision cross section measurements.

Original languageEnglish
Pages (from-to)2013-2022
Number of pages10
JournalJACS Au
Volume2
Issue number9
Early online date7 Sept 2022
DOIs
Publication statusPublished - 26 Sept 2022

Keywords

  • biomarker classes
  • ion mobility
  • lipids
  • paper spray ionization
  • Parkinson's disease
  • sebum

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