Pentapeptide scanning mutagenesis: Encouraging old proteins to execute unusual tricks

Finbarr Hayes; Bernard Hallet

doi:10.1016/S0966-842X(00)01857-6

Pentapeptide scanning mutagenesis: Encouraging old proteins to execute unusual tricks

Finbarr Hayes, Bernard Hallet

Research output: Contribution to journal › Article › peer-review

Abstract

Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity. Copyright (C) 2000 Elsevier Science Ltd.

Original language	English
Pages (from-to)	571-577
Number of pages	6
Journal	Trends in Microbiology
Volume	8
Issue number	12
DOIs	https://doi.org/10.1016/S0966-842X(00)01857-6
Publication status	Published - 1 Dec 2000

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title = "Pentapeptide scanning mutagenesis: Encouraging old proteins to execute unusual tricks",

abstract = "Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity. Copyright (C) 2000 Elsevier Science Ltd.",

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AB - Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity. Copyright (C) 2000 Elsevier Science Ltd.

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DO - 10.1016/S0966-842X(00)01857-6

M3 - Article

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JO - Trends in Microbiology

JF - Trends in Microbiology

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Pentapeptide scanning mutagenesis: Encouraging old proteins to execute unusual tricks

Abstract

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