Peptide hydrogel in-vitro non-inflammatory potential

A Markey, Victoria Workman, Iain Bruce, T J Woolford, Brian Derby, A F Miller, Sarah Cartmell, Alberto Saiani

Research output: Contribution to journalArticlepeer-review


Peptide-based hydrogels have attracted significant interest in recent years as these soft, highly hydrated materials can be engineered to mimic the cell niche with significant potential applications in the biomedical field. Their potential use in vivo in particular is dependent on their biocompatibility, including their potential to cause an inflammatory response. In this work, we investigated in vitro the inflammatory potential of a β-sheet forming peptide (FEFEFKFK; F: phenylalanine, E: glutamic acid; K: lysine) hydrogel by encapsulating murine monocytes within it (3D culture) and using the production of cytokines, IL-β, IL-6 and TNFα, as markers of inflammatory response. No statistically significant release of cytokines in our test sample (media + gel + cells) was observed after 48 or 72 h of culture showing that our hydrogels do not incite a pro-inflammatory response in vitro. These results show the potential biocompatibility of these hydrogels and therefore their potential for in vivo use. The work also highlighted the difference in monocyte behaviour, proliferation and morphology changes when cultured in 2D vs. 3D.
Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalBio-medical materials and engineering
Issue number2
Early online date19 Dec 2016
Publication statusPublished - 17 Feb 2017


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