Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices

Research output: Chapter in Book/Conference proceedingChapterpeer-review

Abstract

The ability to record ensemble action potential (AP) discharge frequencies from large populations of neurons over extended periods of time in vitro offers clear advantages in neuroscience and circadian biology research. Here, we provide an overview of a step-by-step method to perform multisite extracellular AP activity recordings in suprachiasmatic and mediobasal hypothalamic nuclei brain slices, using a state-of-the-art perforated multielectrode array system. Further, we describe in detail a setup architecture which systematically delivers stable, high-quality recordings with excellent anatomical accuracy and consistency. We also provide some procedural, technical, and methodological troubleshooting notes and examples of good quality recordings.

Original languageEnglish
Title of host publicationCircadian Clocks
Subtitle of host publicationMethods and Protocols
EditorsSteven A. Brown
Place of PublicationNew York
PublisherHumana Press, Inc
Pages263-285
Number of pages23
ISBN (Electronic)9781071603819
ISBN (Print)9781071603833, 9781071603802
DOIs
Publication statusPublished - 8 Dec 2020

Publication series

NameMethods in molecular biology
PublisherHumana Press, Inc
Volume2130
ISSN (Print)1064-3745

Keywords

  • perforated multielectrode array
  • electrophysiology
  • suprachiasmatic nuclei
  • mediobasal hypothalamus
  • multiunit activity
  • circadian

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