Peripheral axon crush elevates transport of p75NTR in the central projection of sensory neurones of rats

Jean Dominique Delcroix; Jyoti Patel; Sharon Averill; David R. Tomlinson; John V. Priestley; Paul Fernyhough

doi:10.1016/S0304-3940(03)00749-3

Peripheral axon crush elevates transport of p75NTR in the central projection of sensory neurones of rats

Jean Dominique Delcroix, Jyoti Patel, Sharon Averill, David R. Tomlinson, John V. Priestley, Paul Fernyhough

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of peripheral axon crush on the axonal transport of the neurotrophin receptors, p75NTR and trkA, was studied in dorsal roots of adult rats. Lumbar dorsal roots were crushed for 3-6 h to cause accumulation of p75NTR and trkA. Immunohistochemistry showed the presence of the NGF receptors in axons, indicating retrograde and anterograde axonal transport in the dorsal root. Western blots confirmed that the time course of accumulation of p75NTR was consistent with fast axonal transport. However, trkA accumulation was too low to indicate significant levels of axonal transport. Sciatic nerve crush induced a 2-fold increase (P

Original language	English
Pages (from-to)	181-185
Number of pages	4
Journal	Neuroscience letters
Volume	351
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(03)00749-3
Publication status	Published - 20 Nov 2003

Keywords

Axonal transport
Dorsal root
Dorsal root ganglia
Nerve growth factor receptor
Neurotrophin
trkA

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10.1016/S0304-3940(03)00749-3

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title = "Peripheral axon crush elevates transport of p75NTR in the central projection of sensory neurones of rats",

abstract = "The effect of peripheral axon crush on the axonal transport of the neurotrophin receptors, p75NTR and trkA, was studied in dorsal roots of adult rats. Lumbar dorsal roots were crushed for 3-6 h to cause accumulation of p75NTR and trkA. Immunohistochemistry showed the presence of the NGF receptors in axons, indicating retrograde and anterograde axonal transport in the dorsal root. Western blots confirmed that the time course of accumulation of p75NTR was consistent with fast axonal transport. However, trkA accumulation was too low to indicate significant levels of axonal transport. Sciatic nerve crush induced a 2-fold increase (P",

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author = "Delcroix, {Jean Dominique} and Jyoti Patel and Sharon Averill and Tomlinson, {David R.} and Priestley, {John V.} and Paul Fernyhough",

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T1 - Peripheral axon crush elevates transport of p75NTR in the central projection of sensory neurones of rats

AU - Delcroix, Jean Dominique

AU - Patel, Jyoti

AU - Averill, Sharon

AU - Tomlinson, David R.

AU - Priestley, John V.

AU - Fernyhough, Paul

PY - 2003/11/20

Y1 - 2003/11/20

N2 - The effect of peripheral axon crush on the axonal transport of the neurotrophin receptors, p75NTR and trkA, was studied in dorsal roots of adult rats. Lumbar dorsal roots were crushed for 3-6 h to cause accumulation of p75NTR and trkA. Immunohistochemistry showed the presence of the NGF receptors in axons, indicating retrograde and anterograde axonal transport in the dorsal root. Western blots confirmed that the time course of accumulation of p75NTR was consistent with fast axonal transport. However, trkA accumulation was too low to indicate significant levels of axonal transport. Sciatic nerve crush induced a 2-fold increase (P

AB - The effect of peripheral axon crush on the axonal transport of the neurotrophin receptors, p75NTR and trkA, was studied in dorsal roots of adult rats. Lumbar dorsal roots were crushed for 3-6 h to cause accumulation of p75NTR and trkA. Immunohistochemistry showed the presence of the NGF receptors in axons, indicating retrograde and anterograde axonal transport in the dorsal root. Western blots confirmed that the time course of accumulation of p75NTR was consistent with fast axonal transport. However, trkA accumulation was too low to indicate significant levels of axonal transport. Sciatic nerve crush induced a 2-fold increase (P

KW - Axonal transport

KW - Dorsal root

KW - Dorsal root ganglia

KW - Nerve growth factor receptor

KW - Neurotrophin

KW - trkA

U2 - 10.1016/S0304-3940(03)00749-3

DO - 10.1016/S0304-3940(03)00749-3

M3 - Article

SN - 0304-3940

VL - 351

SP - 181

EP - 185

JO - Neuroscience letters

JF - Neuroscience letters

IS - 3

ER -

Peripheral axon crush elevates transport of p75NTR in the central projection of sensory neurones of rats

Abstract

Keywords

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