Abstract
The self-diffusion coefficient of a series of DNA fragments ranging from 280 to 5386 bases has been measured by fluorescence recovery after photobleaching after thermal denaturation in 8 M urea. The total persistence length p of single-stranded DNAs and its variation in ionic strength down to 10-3 M has been deduced. The importance of the value of p versus the pore size a and contour length L of the DNA in the optimization of sequencing by gel electrophoresis is emphasized.
| Original language | English |
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| Pages (from-to) | 5763-5765 |
| Number of pages | 2 |
| Journal | Macromolecules |
| Volume | 30 |
| Issue number | 19 |
| Publication status | Published - 22 Sept 1997 |