Persistence length of single-stranded DNA

Bernard Tinland, Alain Pluen, Jean Sturm, Gilbert Weill

    Research output: Contribution to journalArticlepeer-review


    The self-diffusion coefficient of a series of DNA fragments ranging from 280 to 5386 bases has been measured by fluorescence recovery after photobleaching after thermal denaturation in 8 M urea. The total persistence length p of single-stranded DNAs and its variation in ionic strength down to 10-3 M has been deduced. The importance of the value of p versus the pore size a and contour length L of the DNA in the optimization of sequencing by gel electrophoresis is emphasized.
    Original languageEnglish
    Pages (from-to)5763-5765
    Number of pages2
    Issue number19
    Publication statusPublished - 22 Sept 1997


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