pH-induced changes in calcium: Functional consequences and mechanisms of action in guinea pig portal vein

R. D. Smith, D. A. Eisner, Susan Wray

    Research output: Contribution to journalArticlepeer-review


    The effects of changing extracellular (pH o) and intracellular pH (pH i) on force and the mechanisms involved in the guinea pig portal vein were investigated to better understand the control of tone in this vessel. When pH o was altered, the effects on force and calcium were the same irrespective of whether force had been produced spontaneously by high-K depolarization or by norepinephrine; alkalinization increased tone, and acidification reduced it. Because pH o changes also lead to changes in ph i, we determined whether the effects on force could be explained by these induced pH i changes. It was found, however, that only with spontaneous activity did intracellular alkalinization increase force. In depolarized preparations, force was decreased, and, with norepinephrine, force was initially decreased and then increased. Thus the effects of pH o cannot be explained solely by changes in pH i. The role of the sarcoplasmic reticulum (SR) and surface membrane Ca 2+-ATPase on the mechanism were investigated and shown not to be involved. Therefore, it is concluded that both pH o and pH i can have powerful modulatory effects on portal vein tone, that these effects are not identical, and that they are likely to be due to effects of pH on ion channels rather than the SR or plasma membrane Ca 2+-ATPase.
    Original languageEnglish
    Pages (from-to)H2518-H2526
    JournalAmerican Journal of Physiology: Heart and Circulatory Physiology
    Issue number6
    Publication statusPublished - 1 Dec 2002


    • Contraction
    • Force
    • Potassium


    Dive into the research topics of 'pH-induced changes in calcium: Functional consequences and mechanisms of action in guinea pig portal vein'. Together they form a unique fingerprint.

    Cite this