Phosphorylation and membrane dissociation of the ARF exchange factor GBF1 in mitosis

Yuichi Morohashi, Zita Balklava, Matthew Ball, Helen Hughes, Martin Lowe

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Secretory protein trafficking is arrested and the Golgi apparatus fragmented when mammalian cells enter mitosis. These changes are thought to facilitate cell-cycle progression and Golgi inheritance, and are brought about through the actions of mitotically active protein kinases. To better understand how the Golgi apparatus undergoes mitotic fragmentation we have sought to identify novel Golgi targets for mitotic kinases. We report in the present paper the identification of the ARF (ADP-ribosylation factor) exchange factor GBF1 (Golgi-specific brefeldin A-resistant guanine nucleotide-exchange factor 1) as a Golgi phosphoprotein. GBF1 is phosphorylated by CDK1 (cyclindependent kinase 1)-cyclin B in mitosis, which results in its dissociation from Golgi membranes. Consistent with a reduced level of GBF1 activity at the Golgi membrane there is a reduction in levels of membrane-associated GTP-bound ARF in mitotic cells. Despite the reduced levels of membrane-bound GBF1 and ARF, COPI (coat protein I) binding to the Golgi membrane appears unaffected in mitotic cells. Surprisingly, this pool of COPI is dependent upon GBF1 for its recruitment to the membrane, suggesting that a low level of GBF1 activity persists in mitosis. We propose that the phosphorylation and membrane dissociation of GBF1 and the consequent reduction in ARF-GTP levels in mitosis are important for changes in Golgi dynamics and possibly other mitotic events mediated through effectors other than the COPI vesicle coat. © The Authors.
    Original languageEnglish
    Pages (from-to)401-412
    Number of pages11
    JournalBiochemical Journal
    Volume427
    Issue number3
    DOIs
    Publication statusPublished - 1 May 2010

    Keywords

    • ADP-ribosylation factor (ARF)
    • Golgi apparatus
    • Golgi-specific brefeldin A-resistant guanine nucleotide-exchange factor 1 (GBF1)
    • Mitosis
    • Phosphorylation

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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