Photolabelling of dopamine-β-hydroxylase by bleomycin

Hiran A.M. Ratwatte; Kenneth T. Douglas

doi:10.1016/0006-291X(83)91826-0

Photolabelling of dopamine-β-hydroxylase by bleomycin

Hiran A.M. Ratwatte, Kenneth T. Douglas^*

^*Corresponding author for this work

Pharmacy

The University of Essex

Research output: Contribution to journal › Article › peer-review

Abstract

Irradiation of a mixture of dopamine-β-hydroxylase and bleomycin with light of wavelengths >300 nm. leads to irreversible inhibition which is not prevented in the presence of tyramine. Prephotolysis of bleomycin followed by incubation (in the dark) with dopamine-β-hydroxylase did not lead to irreversible inhibition. Thus bleomycin is a photolabel of this enzyme.

Original language	English
Pages (from-to)	273-278
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	112
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(83)91826-0
Publication status	Published - 15 Apr 1983

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title = "Photolabelling of dopamine-β-hydroxylase by bleomycin",

abstract = "Irradiation of a mixture of dopamine-β-hydroxylase and bleomycin with light of wavelengths >300 nm. leads to irreversible inhibition which is not prevented in the presence of tyramine. Prephotolysis of bleomycin followed by incubation (in the dark) with dopamine-β-hydroxylase did not lead to irreversible inhibition. Thus bleomycin is a photolabel of this enzyme.",

author = "Ratwatte, {Hiran A.M.} and Douglas, {Kenneth T.}",

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AU - Ratwatte, Hiran A.M.

AU - Douglas, Kenneth T.

PY - 1983/4/15

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N2 - Irradiation of a mixture of dopamine-β-hydroxylase and bleomycin with light of wavelengths >300 nm. leads to irreversible inhibition which is not prevented in the presence of tyramine. Prephotolysis of bleomycin followed by incubation (in the dark) with dopamine-β-hydroxylase did not lead to irreversible inhibition. Thus bleomycin is a photolabel of this enzyme.

AB - Irradiation of a mixture of dopamine-β-hydroxylase and bleomycin with light of wavelengths >300 nm. leads to irreversible inhibition which is not prevented in the presence of tyramine. Prephotolysis of bleomycin followed by incubation (in the dark) with dopamine-β-hydroxylase did not lead to irreversible inhibition. Thus bleomycin is a photolabel of this enzyme.

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U2 - 10.1016/0006-291X(83)91826-0

DO - 10.1016/0006-291X(83)91826-0

M3 - Article

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AN - SCOPUS:0020586875

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SP - 273

EP - 278

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Photolabelling of dopamine-β-hydroxylase by bleomycin

Abstract

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