Photopatterning of self assembled monolayers on oxide surfaces for the selective attachment of biomolecules

Pompi Hazarika, Jonathan M. Behrendt, Linn Petersson, Christer Wingren, Michael L. Turner

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum. © 2013 Elsevier B.V.
    Original languageEnglish
    Pages (from-to)82-89
    Number of pages7
    JournalBiosensors and Bioelectronics
    Volume53
    DOIs
    Publication statusPublished - 15 Mar 2014

    Keywords

    • Biomarker detection
    • Photo-pattern
    • Proteins
    • Self-assembled monolayer (SAM)
    • Silicon

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