Abstract
The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum. © 2013 Elsevier B.V.
Original language | English |
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Pages (from-to) | 82-89 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 53 |
DOIs | |
Publication status | Published - 15 Mar 2014 |
Keywords
- Biomarker detection
- Photo-pattern
- Proteins
- Self-assembled monolayer (SAM)
- Silicon