Phylogenetic analysis of complete 5′ external transcribed spacers of the 18S ribosomal RNA genes of diploid Aegilops and related species (Triticeae, Poaceae)

PCR systems were designed to amplify the entire 5′ external transcribed spacer (ETS) region of the 18S rRNA gene of all the diploid species of Aegilops and several other taxa closely related to domesticated wheat. Phylogenetic analysis was performed on the complete ETS sequences using the neighbor-joining, maximum parsimony, and maximum likelihood methods. Among the individual taxa studied, speciation in Secale is very recent. In the case of the A genome diploids, the results support the theory that the A genomes of wheat have experienced reticulate evolution owing to introgression. The B and G genomes of tetraploid domesticated wheats form a clade with Ae. speltoides in which the B genome diverged first and the G genome more recently. It was demonstrated that the complete ETS sequences of the Triticeae yield coherent phylogenetic information. The ETS is a useful tool for studying the phylogeny of closely related species.