Phylogenetic relationships of methionine aminopeptidase 2 among Encephalitozoon species and genotypes of microsporidia

Ivona Pandrea, Derek Mittleider, Paul J. Brindley, Elizabeth S. Didier, David L. Robertson

    Research output: Contribution to journalArticlepeer-review

    Abstract

    This report describes the characterization and phylogenetic analysis of the deduced amino acid sequences of methionine aminopeptidase 2 (MetAP-2) enzymes from microsporidian species and genotypes of the genus Encephalitozoon. Fragments of DNA encoding 318 to 335 amino acid residues of the MetAP-2 genes were isolated from genomic DNA prepared from cultured spores of Encephalitozoon hellem, Encephalitozoon intestinalis, and Encephalitozoon cuniculi genotypes I-III. Sequence comparisons of the deduced amino acid residues indicated that the microsporidian sequences are MetAP-2-like rather than MetAP-1-like. Alignments demonstrated that the new Encephalitozoon sequences included sequences and structures conserved in eukaryotic MetAP-2s, including the five conserved, active site residues, Asp, Asp, His, Glu, and His, considered to be critical for catalysis and for coordinating the cation (e.g., cobalt) co-factor, and included residues known to interact with the antibiotic, fumagillin. The primary structure of the Encephalitozoon MetAP-2s, however, showed some dissimilarity with human and yeast MetAP-2s, including the absence of the NH2-terminal polylysine tract. Phylogenetic comparison of these Encephalitozoon MetAP-2s with orthologues from related species and from other informative taxa confirmed that the MetAP-2s of these Encephalitozoon species and strains are closely related to each other and cluster with MetAP-2s. © 2004 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)141-152
    Number of pages11
    JournalMolecular and biochemical parasitology
    Volume140
    Issue number2
    DOIs
    Publication statusPublished - Apr 2005

    Keywords

    • Emerging infectious disease
    • Encephalitozoon
    • Fumagillin
    • Methionine aminopeptidase 2
    • Microsporidiosis
    • Molecular phylogeny
    • Opportunistic pathogen

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