Abstract
Purpose: The Popeye domain containing (Popdc) family of three novel muscle-restricted genes (Popdc1-3) are evolutionarily conserved and expressed prominently within the cardiac conduction system and the purpose of this study was to delineate the role of Popdc1 in sinus node pacemaking using transgenic mice lacking the Popdc1 gene (Popdc1-/-).
Methods: Male Popdc1-/- mice (C57BL/6J background) of 5.5 months age were used. The study followed "Principles of laboratory animal care" (NIH Publication no. 85-23 revised 1985) and is in accordance with the United Kingdom Animals (Scientific Procedures) Act, 1986. The ECG in conscious animals was recorded via telemetry at rest, during and after physiological stress (swim) or catecholamine treatment. In addition, extracellular potentials were recorded from isolated sinus node preparations.
Methods: Male Popdc1-/- mice (C57BL/6J background) of 5.5 months age were used. The study followed "Principles of laboratory animal care" (NIH Publication no. 85-23 revised 1985) and is in accordance with the United Kingdom Animals (Scientific Procedures) Act, 1986. The ECG in conscious animals was recorded via telemetry at rest, during and after physiological stress (swim) or catecholamine treatment. In addition, extracellular potentials were recorded from isolated sinus node preparations.
| Original language | English |
|---|---|
| Pages (from-to) | 2290 |
| Journal | European Heart Journal |
| Volume | 34 |
| Issue number | Supplement 1 |
| Publication status | Published - Aug 2013 |
Keywords
- Cardiac arrhythmia
- artificial cardiac pacemaker
- phenotype
- isoproterenol
- Catecholamines