Positron emission tomography to visualise in-situ microbial metabolism in natural sediments

Clare L. Thorpe, Heather A. Williams, Christopher Boothman, Jonathan R. Lloyd, Katherine Morris

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Abstract

Positron Emission Tomography simultaneously monitored flow dynamics and in-situ microbial activity in opaque sediment columns. An 18F labelled electron donor, 2-deoxy-2-[18F]-fluoro-D-glucose (18FDG), was added to flowing columns containing sterile sediment, microbially active sediment and quartz sand. 18FDG accumulated in microbially active, glucose primed, sediment (105–106 cells g-1) at 10–16 mol g-1 (~40% of the 100 MBq spike) giving the first example of 18FDG uptake by indigenous microorganisms in a complex sediment system at flow rates representative of the shallow subsurface.
Original languageEnglish
JournalApplied Radiation and Isotopes
Early online date20 Nov 2018
DOIs
Publication statusPublished - 2018

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