Potency and risk assessment of a skin-sensitizing disperse dye using the local lymph node assay

Catherine J. Betts, Rebecca J. Dearman, Ian Kimber, Howard I. Maibach

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Disperse Blue 106 is an acknowledged skin-sensitization hazard. However, information about the relative sensitization potency of this chemical is lacking, and to provide this information was the purpose of the investigations described here. The approach taken was to measure dose-response relationships for C.I. Disperse Blue in the local lymph node assay, a method for the assessment of skin-sensitization potential in which activity is measured as a function of lymphocyte proliferative responses induced in draining lymph nodes. From these data, it was possible to derive EC3 values (such being the estimated concentration of chemical required to elicit a 3-fold increase in proliferation) that have been shown previously to reflect the relative sensitizing potency of contact allergens. These analyses revealed that Disperse Blue 106 had a relatively low EC3 value (0.01%), comparable to that measured concurrently for 2,4-dinitrochlorobenzene, a potent contact allergen. Collectively, these data reveal that Disperse Blue 106 represents a significant skin-sensitization hazard, and, in combination with information on dye migration and percutaneous penetration from various types of fabric and use conditions provide a basis for the development of effective and accurate risk assessments. © Blackwell Munksgaard, 2005.
    Original languageEnglish
    Pages (from-to)268-272
    Number of pages4
    JournalContact dermatitis
    Volume52
    Issue number5
    DOIs
    Publication statusPublished - May 2005

    Keywords

    • Dinitrochlorobenzene
    • Disperse Blue 106
    • Local lymph node assay
    • Risk assessment
    • Skin-sensitization potency

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