Practical Aspects of the Measurement of the Diffusion of Proteins in Aqueous Solution

Marcus L. Tillett; Lu Yun Lian; Timothy J. Norwood

Practical Aspects of the Measurement of the Diffusion of Proteins in Aqueous Solution

Marcus L. Tillett, Lu Yun Lian, Timothy J. Norwood

Research output: Contribution to journal › Article › peer-review

Abstract

NMR diffusion measurements have been shown to be a useful tool for investigating the conformation and oligomeric state of proteins. Four main problems associated with making diffusion measurements of proteins in aqueous solution are identified and solutions proposed. The resulting experiment is demonstrated for an aqueous solution of hen egg white lysozyme. © 1998 Academic Press.

Original language	English
Pages (from-to)	379-384
Number of pages	5
Journal	JOURNAL OF MAGNETIC RESONANCE
Volume	133
Issue number	2
Publication status	Published - Aug 1998

Keywords

Diffusion
Lysozyme
PGSE
Proteins

Cite this

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title = "Practical Aspects of the Measurement of the Diffusion of Proteins in Aqueous Solution",

abstract = "NMR diffusion measurements have been shown to be a useful tool for investigating the conformation and oligomeric state of proteins. Four main problems associated with making diffusion measurements of proteins in aqueous solution are identified and solutions proposed. The resulting experiment is demonstrated for an aqueous solution of hen egg white lysozyme. {\textcopyright} 1998 Academic Press.",

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author = "Tillett, {Marcus L.} and Lian, {Lu Yun} and Norwood, {Timothy J.}",

year = "1998",

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pages = "379--384",

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T1 - Practical Aspects of the Measurement of the Diffusion of Proteins in Aqueous Solution

AU - Tillett, Marcus L.

AU - Lian, Lu Yun

AU - Norwood, Timothy J.

PY - 1998/8

Y1 - 1998/8

N2 - NMR diffusion measurements have been shown to be a useful tool for investigating the conformation and oligomeric state of proteins. Four main problems associated with making diffusion measurements of proteins in aqueous solution are identified and solutions proposed. The resulting experiment is demonstrated for an aqueous solution of hen egg white lysozyme. © 1998 Academic Press.

AB - NMR diffusion measurements have been shown to be a useful tool for investigating the conformation and oligomeric state of proteins. Four main problems associated with making diffusion measurements of proteins in aqueous solution are identified and solutions proposed. The resulting experiment is demonstrated for an aqueous solution of hen egg white lysozyme. © 1998 Academic Press.

KW - Diffusion

KW - Lysozyme

KW - PGSE

KW - Proteins

M3 - Article

C2 - 9716483

SN - 1096-0856

VL - 133

SP - 379

EP - 384

JO - JOURNAL OF MAGNETIC RESONANCE

JF - JOURNAL OF MAGNETIC RESONANCE

IS - 2

ER -

Practical Aspects of the Measurement of the Diffusion of Proteins in Aqueous Solution

Abstract

Keywords

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