Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study

Natalie Finch; Benita Percival; Elena Hunter; Robin J. Blagg; Emily Blackwell; James Sagar; Zeeshan Ahmad; Ming Wei Chang; John A. Hunt; Melissa L. Mather; Séverine Tasker; Luisa De Risio; Philippe B. Wilson

doi:10.1186/s13104-021-05888-y

Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study

Natalie Finch
, Benita Percival
, Elena Hunter
, Robin J. Blagg
, Emily Blackwell
, James Sagar
, Zeeshan Ahmad
, Ming Wei Chang
, John A. Hunt
, Melissa L. Mather
, Séverine Tasker
, Luisa De Risio
, Philippe B. Wilson^*

^*Corresponding author for this work

Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making. Results: Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.

Original language	English
Article number	469
Journal	BMC Research Notes
Volume	14
Issue number	1
DOIs	https://doi.org/10.1186/s13104-021-05888-y
Publication status	Published - Dec 2021

Keywords

Cat
Chronic kidney disease
Metabolite
Metabolomics
NMR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/s13104-021-05888-y

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Finch, N., Percival, B., Hunter, E., Blagg, R. J., Blackwell, E., Sagar, J., Ahmad, Z., Chang, M. W., Hunt, J. A., Mather, M. L., Tasker, S., De Risio, L., & Wilson, P. B. (2021). Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study. BMC Research Notes, 14(1), Article 469. https://doi.org/10.1186/s13104-021-05888-y

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title = "Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study",

abstract = "Objective: The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making. Results: Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.",

keywords = "Cat, Chronic kidney disease, Metabolite, Metabolomics, NMR",

author = "Natalie Finch and Benita Percival and Elena Hunter and Blagg, \{Robin J.\} and Emily Blackwell and James Sagar and Zeeshan Ahmad and Chang, \{Ming Wei\} and Hunt, \{John A.\} and Mather, \{Melissa L.\} and S{\'e}verine Tasker and \{De Risio\}, Luisa and Wilson, \{Philippe B.\}",

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month = dec,

doi = "10.1186/s13104-021-05888-y",

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T2 - chronic kidney disease as a case study

AU - Finch, Natalie

AU - Percival, Benita

AU - Hunter, Elena

AU - Blagg, Robin J.

AU - Blackwell, Emily

AU - Sagar, James

AU - Ahmad, Zeeshan

AU - Chang, Ming Wei

AU - Hunt, John A.

AU - Mather, Melissa L.

AU - Tasker, Séverine

AU - De Risio, Luisa

AU - Wilson, Philippe B.

PY - 2021/12

Y1 - 2021/12

N2 - Objective: The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making. Results: Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.

AB - Objective: The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making. Results: Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.

KW - Cat

KW - Chronic kidney disease

KW - Metabolite

KW - Metabolomics

KW - NMR

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DO - 10.1186/s13104-021-05888-y

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AN - SCOPUS:85121726174

SN - 1756-0500

VL - 14

JO - BMC Research Notes

JF - BMC Research Notes

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ER -

Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study

Abstract

Keywords

UN SDGs

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