Pressurized CO2 as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol

Katharina Plasch; Gerhard Hofer; Walter Keller; Sam Hay; Derren Heyes; Alexander Dennig; Silvia M.  Glueck; Kurt Faber

doi:10.1039/C8GC00008E

Pressurized CO₂as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol

Katharina Plasch, Gerhard Hofer, Walter Keller, Sam Hay, Derren Heyes, Alexander Dennig, Silvia M. Glueck, Kurt Faber

Research output: Contribution to journal › Article › peer-review

Abstract

The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO₂ (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

Original language	English
Journal	Green Chemistry
Early online date	3 Apr 2018
DOIs	https://doi.org/10.1039/C8GC00008E
Publication status	Published - 2018

Keywords

biocatalysis
ortho-carboxylation
ortho-benzoic acid decarboxylases
pressurized CO2

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

Access to Document

10.1039/C8GC00008ELicence: CC BY

Biochemical and Biophysical Sciences Technology Platform
Heyes, D. (Senior Technical Specialist), Boothman, C. (Senior Technical Specialist), Cliffe, L. (Technical Specialist), Dunstan, M. (Senior Technical Specialist), Golovanova, M. (Senior Technician), Hoeven, R. (Technical Specialist), Lopez Perez, R. (Senior Technician), Sakuma, M. (Senior Technician), Tait, S. (Senior Technician) & Tilakaratna, V. (Senior Technician)
FSE Research
Facility/equipment: Platform

Cite this

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title = "Pressurized CO2 as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol",

abstract = "The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.",

keywords = "biocatalysis, ortho-carboxylation, ortho-benzoic acid decarboxylases, pressurized CO2",

author = "Katharina Plasch and Gerhard Hofer and Walter Keller and Sam Hay and Derren Heyes and Alexander Dennig and Glueck, {Silvia M.} and Kurt Faber",

year = "2018",

doi = "10.1039/C8GC00008E",

language = "English",

journal = "Green Chemistry",

issn = "1463-9262",

publisher = "Royal Society of Chemistry",

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T1 - Pressurized CO2 as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol

AU - Plasch, Katharina

AU - Hofer, Gerhard

AU - Keller, Walter

AU - Hay, Sam

AU - Heyes, Derren

AU - Dennig, Alexander

AU - Glueck, Silvia M.

AU - Faber, Kurt

PY - 2018

Y1 - 2018

N2 - The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

AB - The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

KW - biocatalysis

KW - ortho-carboxylation

KW - ortho-benzoic acid decarboxylases

KW - pressurized CO2

U2 - 10.1039/C8GC00008E

DO - 10.1039/C8GC00008E

M3 - Article

SN - 1463-9262

JO - Green Chemistry

JF - Green Chemistry

ER -