Abstract
The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.
Original language | English |
---|---|
Journal | Green Chemistry |
Early online date | 3 Apr 2018 |
DOIs | |
Publication status | Published - 2018 |
Keywords
- biocatalysis
- ortho-carboxylation
- ortho-benzoic acid decarboxylases
- pressurized CO2
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology
Fingerprint
Dive into the research topics of 'Pressurized CO2 as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol'. Together they form a unique fingerprint.Equipment
-
Biochemical and Biophysical Sciences Technology Platform
Heyes, D. (Senior Technical Specialist), Boothman, C. (Senior Technical Specialist), Cliffe, L. (Technical Specialist), Dunstan, M. (Senior Technical Specialist), Golovanova, M. (Senior Technician), Hoeven, R. (Technical Specialist), Lopez Perez, R. (Senior Technician), Sakuma, M. (Senior Technician), Tait, S. (Senior Technician) & Tilakaratna, V. (Senior Technician)
FSE ResearchFacility/equipment: Platform