Pressurized CO2 as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol

Katharina Plasch, Gerhard Hofer, Walter Keller, Sam Hay, Derren Heyes, Alexander Dennig, Silvia M. Glueck, Kurt Faber

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.
    Original languageEnglish
    JournalGreen Chemistry
    Early online date3 Apr 2018
    DOIs
    Publication statusPublished - 2018

    Keywords

    • biocatalysis
    • ortho-carboxylation
    • ortho-benzoic acid decarboxylases
    • pressurized CO2

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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    • Biochemical and Biophysical Sciences Technology Platform

      Heyes, D. (Senior Technical Specialist), Boothman, C. (Senior Technical Specialist), Cliffe, L. (Technical Specialist), Dunstan, M. (Senior Technical Specialist), Golovanova, M. (Senior Technician), Hoeven, R. (Technical Specialist), Lopez Perez, R. (Senior Technician), Sakuma, M. (Senior Technician), Tait, S. (Senior Technician) & Tilakaratna, V. (Senior Technician)

      FSE Research

      Facility/equipment: Platform

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