Pressurized CO2 as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol

Katharina Plasch; Gerhard Hofer; Walter Keller; Sam Hay; Derren Heyes; Alexander Dennig; Silvia M.  Glueck; Kurt Faber

doi:10.1039/C8GC00008E

Pressurized CO₂as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol

Katharina Plasch, Gerhard Hofer, Walter Keller, Sam Hay, Derren Heyes, Alexander Dennig, Silvia M. Glueck, Kurt Faber

Research output: Contribution to journal › Article › peer-review

Abstract

The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO₂ (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

Original language	English
Journal	Green Chemistry
Early online date	3 Apr 2018
DOIs	https://doi.org/10.1039/C8GC00008E
Publication status	Published - 2018

Keywords

biocatalysis
ortho-carboxylation
ortho-benzoic acid decarboxylases
pressurized CO2

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

Access to Document

10.1039/C8GC00008ELicence: CC BY

Cite this

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title = "Pressurized CO2 as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol",

abstract = "The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.",

keywords = "biocatalysis, ortho-carboxylation, ortho-benzoic acid decarboxylases, pressurized CO2",

author = "Katharina Plasch and Gerhard Hofer and Walter Keller and Sam Hay and Derren Heyes and Alexander Dennig and Glueck, {Silvia M.} and Kurt Faber",

year = "2018",

doi = "10.1039/C8GC00008E",

language = "English",

journal = "Green Chemistry",

issn = "1463-9262",

publisher = "Royal Society of Chemistry",

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T1 - Pressurized CO2 as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol

AU - Plasch, Katharina

AU - Hofer, Gerhard

AU - Keller, Walter

AU - Hay, Sam

AU - Heyes, Derren

AU - Dennig, Alexander

AU - Glueck, Silvia M.

AU - Faber, Kurt

PY - 2018

Y1 - 2018

N2 - The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

AB - The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on large scale. As proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (~30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

KW - biocatalysis

KW - ortho-carboxylation

KW - ortho-benzoic acid decarboxylases

KW - pressurized CO2

U2 - 10.1039/C8GC00008E

DO - 10.1039/C8GC00008E

M3 - Article

SN - 1463-9262

JO - Green Chemistry

JF - Green Chemistry

ER -