Prevalence and multiplicity of cutaneous beta papilloma viruses in plucked hairs depend on cellular DNA input

Adele Green, S. J. Weissenborn, R. Neale, M. N C de Koning, T. Waterboer, D. Abeni, J. N. Bouwes Bavinck, U. Wieland, H. J. Pfister

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In view of the low loads of beta human papillomaviruses in skin samples, amounts of cellular DNA used in qualitative PCR may become limiting for virus detection and introduce variations in prevalence and multiplicity. This issue was explored within the context of a multicentre study and increasing prevalence and multiplicity was found with increasing input amounts of cellular DNA extracted from hair bulbs. To improve the quality and comparability between different epidemiologic studies ideally equal amounts of cellular DNA should be employed. When cellular DNA input varies this should be clearly taken into account in assessing viral prevalence and multiplicity. © 2009 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)280-283
    Number of pages3
    JournalJournal of Virological Methods
    Volume161
    Issue number2
    DOIs
    Publication statusPublished - Nov 2009

    Keywords

    • Beta papillomavirus
    • Cellular DNA
    • Detection
    • Epidemiology
    • HPV
    • Human papillomavirus
    • Multiplicity
    • PCR
    • Prevalence

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